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EC number: 700-941-3 | CAS number: -
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cuprate(4-), [2-[[4-chloro-6-[ethyl[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-5-(hydroxy-kO)-6-[2-[2-[2-[2-(hydroxy-kO)-5-sulfophenyl]diazenyl-kN1]-4,5-dimethoxyphenyl]diazenyl-kN2]-1,7-naphthalenedisulfonato(6-)]-, hydrogen sodium (1:2:2)
- EC Number:
- 700-941-3
- Cas Number:
- 1512859-54-9
- Molecular formula:
- C37H28ClCuN9O19S5 . 2 Na . 2 H
- IUPAC Name:
- Cuprate(4-), [2-[[4-chloro-6-[ethyl[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-5-(hydroxy-kO)-6-[2-[2-[2-[2-(hydroxy-kO)-5-sulfophenyl]diazenyl-kN1]-4,5-dimethoxyphenyl]diazenyl-kN2]-1,7-naphthalenedisulfonato(6-)]-, hydrogen sodium (1:2:2)
Constituent 1
Test organisms
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- Source Friedrich-schiller-universität Jena
Dornburger Straße 159
D-07743 Jena, Germany
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Test duration 7 days
Test type Semi-static, with renewal of test solutions on days 3 and 5
Test vessels 250 ml, all-glass, containing 150 ml of test solution
Medium Modified Steinberg medium
Initial plant density 3 plants, with a total of 9 fronds
Test conditions
- Test temperature:
- 22-26 ºC
- pH:
- 5.5
- Nominal and measured concentrations:
- 0.1, 1, 10 and 100 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 98 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 98 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- meas. (TWA)
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- Under the conditions of the reference study with Lemna minor, 3,5-dichlorophenol reduced growth rate of this aquatic plant at nominally 0.56 mg/l and higher.
The EC50 for growth rate reduction (ERC50: 0-7d) was 2.4 mg/l with a 95% confidence interval ranging from 1.4 to 4.2 mg/l. According to ISO 20079 the ranges for growth rate reduction should lie between 2.2 and 3.8 mg/l. Hence, the ERC50: 0-7d for the present batch corresponds with this range.
The EC50 for yield inhibition (EYC50: 0-7d) was 2.1 mg/l with a 95% confidence interval ranging from 1.2 to 3.8 mg/l. Historical ranges are not yet available.
Any other information on results incl. tables
Effect parameters
Parameter |
Concentration Everzol SB46 (mg/l) |
95%-confidence interval |
NOEC (growth rate, frond numbers) |
9.8 |
|
LOEC (growth rate, frond numbers) |
30 |
|
7d-ERC50 (frond numbers) |
>98 |
|
NOEC (growth rate, wet weight) |
30 |
|
LOEC (growth rate, wet weight) |
98 |
|
7d-ERC50 (wet weight) |
>98 |
|
NOEC (yield, frond numbers) |
9.8 |
|
LOEC (yield, frond numbers) |
30 |
|
7d-EYC50 (frond numbers) |
77 |
8.2 - 726 |
NOEC (yield, wet weight) |
30 |
|
LOEC (yield, wet weight) |
98 |
|
7d-EYC50 (wet weight) |
76 |
7.4 - 781 |
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of the present study with Lemna minor, Everzol SB46 reduced growth rate and inhibited the yield of this fresh water plant species significantly at 30 mg/l (LOEC). The NOEC for growth rate reduction and yield inhibition was 8.9 mg/l.
The EC50 for growth rate reduction (ERC50: 0-7d) for frond numbers and wet weight was beyond the rage tested, i.e. exceeded the average exposure concentration of 98 mg/l.
The EC50 for yield inhibition (EYC50: 0-7d) based on frond numbers and wet weight were similar, i.e. 77 and 76 mg/l, respectively.
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