Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic plants other than algae

Administrative data

Endpoint:
toxicity to aquatic plants other than algae
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Cuprate(4-), [2-[[4-chloro-6-[ethyl[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-5-(hydroxy-kO)-6-[2-[2-[2-[2-(hydroxy-kO)-5-sulfophenyl]diazenyl-kN1]-4,5-dimethoxyphenyl]diazenyl-kN2]-1,7-naphthalenedisulfonato(6-)]-, hydrogen sodium (1:2:2)
EC Number:
700-941-3
Cas Number:
1512859-54-9
Molecular formula:
C37H28ClCuN9O19S5 . 2 Na . 2 H
IUPAC Name:
Cuprate(4-), [2-[[4-chloro-6-[ethyl[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-5-(hydroxy-kO)-6-[2-[2-[2-[2-(hydroxy-kO)-5-sulfophenyl]diazenyl-kN1]-4,5-dimethoxyphenyl]diazenyl-kN2]-1,7-naphthalenedisulfonato(6-)]-, hydrogen sodium (1:2:2)

Test organisms

Test organisms (species):
Lemna minor
Details on test organisms:
Source Friedrich-schiller-universität Jena
Dornburger Straße 159
D-07743 Jena, Germany

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
7 d
Post exposure observation period:
Test duration 7 days

Test type Semi-static, with renewal of test solutions on days 3 and 5

Test vessels 250 ml, all-glass, containing 150 ml of test solution

Medium Modified Steinberg medium

Initial plant density 3 plants, with a total of 9 fronds

Test conditions

Test temperature:
22-26 ºC
pH:
5.5
Nominal and measured concentrations:
0.1, 1, 10 and 100 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
> 98 mg/L
Nominal / measured:
meas. (TWA)
Basis for effect:
growth rate
Duration:
7 d
Dose descriptor:
LOEC
Effect conc.:
98 mg/L
Nominal / measured:
meas. (TWA)
Basis for effect:
growth rate
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
30 mg/L
Nominal / measured:
meas. (TWA)
Basis for effect:
growth rate
Results with reference substance (positive control):
Under the conditions of the reference study with Lemna minor, 3,5-dichlorophenol reduced growth rate of this aquatic plant at nominally 0.56 mg/l and higher.

The EC50 for growth rate reduction (ERC50: 0-7d) was 2.4 mg/l with a 95% confidence interval ranging from 1.4 to 4.2 mg/l. According to ISO 20079 the ranges for growth rate reduction should lie between 2.2 and 3.8 mg/l. Hence, the ERC50: 0-7d for the present batch corresponds with this range.

The EC50 for yield inhibition (EYC50: 0-7d) was 2.1 mg/l with a 95% confidence interval ranging from 1.2 to 3.8 mg/l. Historical ranges are not yet available.

Any other information on results incl. tables

Effect parameters

Parameter

Concentration Everzol SB46

(mg/l)

95%-confidence interval

NOEC (growth rate, frond numbers)

9.8

 

LOEC (growth rate, frond numbers)

30

 

7d-ERC50 (frond numbers)

>98

 

NOEC (growth rate, wet weight)

30

 

LOEC (growth rate, wet weight)

98

 

7d-ERC50 (wet weight)

>98

 

NOEC (yield, frond numbers)

9.8

 

LOEC (yield, frond numbers)

30

 

7d-EYC50 (frond numbers)

77

8.2 - 726

NOEC (yield, wet weight)

30

 

LOEC (yield, wet weight)

98

 

7d-EYC50 (wet weight)

76

7.4 - 781

Applicant's summary and conclusion

Conclusions:
Under the conditions of the present study with Lemna minor, Everzol SB46 reduced growth rate and inhibited the yield of this fresh water plant species significantly at 30 mg/l (LOEC). The NOEC for growth rate reduction and yield inhibition was 8.9 mg/l.

The EC50 for growth rate reduction (ERC50: 0-7d) for frond numbers and wet weight was beyond the rage tested, i.e. exceeded the average exposure concentration of 98 mg/l.

The EC50 for yield inhibition (EYC50: 0-7d) based on frond numbers and wet weight were similar, i.e. 77 and 76 mg/l, respectively.

Categories Display