3,4,5-trimethoxytoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): 3,4,5-Trimethoxytoluol
- Physical state: yellowish, solid
- Analytical purity: 96.6%
- Lot/batch No.: 30364/143
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 12.5, 25, 50, 100 mg/L
- Sampling method: at the begin (after 0 h) and at the end of the exposition (after 48 h) samples from vessels without daphnids were analysed

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The concentration of the stock solution was 100 mg/L (net weight : 50.3 mg/500 ml). The stock solution was treated in an ultrasonic bath for approx. 15 min and then stirred for approx. 30 min

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology
- Age at study initiation: 2 - 24 h (starting with the 3rd breed of parent animals)
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.42 mmol/L

Test temperature:

19.5 - 20.1 °C

pH:

7.8 - 8.1

Dissolved oxygen:

8.5 - 9.3 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 12.5, 25, 50, 100 mg/L
Measured test concentrations (0 h): n.d., 13.4, 24.9, 49.3, 135.9 mg/L
Measured test concentrations (48 h): n.d., 12.5, 24.9, 49.0, 95.8 mg/L
n.d. = non-detectable

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: a synthetic fresh water was used for culture and test purposes (M4 medium)
- Alkalinity: 0.87 mmol/L
- Conductivity: 608 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement:
- Temperature: Continuously during the whole test period in a separate vessel close the test vessels
- pH, oxygen content: after 0 h in an additional replicate without daphnids and after 48 h in replicate 1 of each tested concentration

OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm 16:8 h
- Light intensity: artificial light, type warm white; about 1-8 µE/(m²/s) at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED: mobility after 0, 24, 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility