Registration Dossier
Registration Dossier
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EC number: 229-239-0 | CAS number: 6443-69-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): 3,4,5-Trimethoxytoluol
- Physical state: yellowish, solid
- Analytical purity: 96.6%
- Impurities (identity and concentrations): 0.6% 3-Brom-4,5-dimethoxytoluol; 0.15% water
- Lot/batch No.: 30364/143
- Storage condition of test material: room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge from laboratory wastewater plants treating municipal sewage
- Concentration of dry substance: 30 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Initial conc.:
- 31 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1000 ml/vessel contains 326 ml test substance (30.9 mg/L), 5 ml inoculum (30 mg/L dry matter), 13 ml inorganic medium, 656 ml deionized water
- Test temperature: 22 ± 2 °C
- pH: 7.4 - 7.8
SAMPLING
- Sampling frequency: Day 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27, 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Adsorbtion control: yes
- Inhibition control: yes
- Reference substance: yes
- Abiotic control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 90 - 100
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
= 1 after 7 days
= 69 after 14 days
= 96 after 21 days
= 99 after 28 days
BOD5 / COD results
- Results with reference substance:
- 93% after 14 days
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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