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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96-Hour LC50 was determined to be > 0.10 mg/L. Correspondingly the No Observed Effect Concentration was ≥ 0.10 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.1 mg/L

Additional information


A study was performed to assess the acute toxicity of the test material to rain bow trout (Oncorhynchus mykiss). The method followed that described in the OECD Guidelines for Testing of Chemicals No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Directive 92/69/EEC.


Following a preliminary range-finding study and pre-study testing fish were exposed, in 2 groups of 10, to an aqueous dispersion of the test material, at a single concentration of 0.10 mg/L for a period of 96 h under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 h after the start of exposure and then daily throughout the study until termination after 96 h.


The 96-Hour LC50 for the test material to rainbow trout (Oncorhynchus mykiss), based on nominal test concentrations was > 0.10 mg/L and correspondingly the No Observed Effect Concentration was ≥ to 0.10 mg/L.

The test concentration of 0.10 mg/L was the highest attainable test concentration due to the limited solubility of the test material in water and auxiliary solvent, and having due regard for the amount of auxiliary solvent permitted in the test under the OECD Guidelines.

Analysis of the test solutions at 0, 24 and 96 h showed the measured test concentrations to be in excess of the required 80 % of nominal test concentrations and so the results are based on nominal test concentrations only.