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Diss Factsheets
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EC number: 940-786-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- long-term toxicity to fish
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- February 10, 2000- March 2, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 203
- Deviations:
- yes
- Remarks:
- Study was extended to 21 days
- GLP compliance:
- not specified
- Remarks:
- (Not stated, but appears to be conducted similar to GLP studies)
Test material
- Reference substance name:
- LAB
- IUPAC Name:
- LAB
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sample storage conditions before analysis: samples were analyzed immediately and processed within 2 hrs, analytical standards were kept in acetonitrile at 0-4 degrees C.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 40 uL of test compound was dissolved in 22 mL of acetone. 356 mL of MilliQ water was then added. The solution was pumped into a premix flask. Fresh stock solution was prepared daily.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): <100 mg/L
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Agrojardin, Avda. Cantabria 4, San Sebastian de los Reyes, 28050 Madrid, Spain
- Length at study initiation (length definition, mean, range and SD): 2 +/- 1 cm
- Feeding during test
- Food type: Nutrafin
- Amount: 2% mean bodyweight
- Frequency: once every three days
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): water temperature, photoperiod and feed were similar to those in test
- Type and amount of food: Nutrafin, 10% of bodyweight
- Feeding frequency: once a day every working day
- Health during acclimation (any mortality observed): no mortality observed
Study design
- Test type:
- flow-through
- Water media type:
- not specified
- Limit test:
- yes
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 49.52-60.95 mg CaCO3/L; determined twice weekly
- Test temperature:
- 20 +/- 1 degree C; determined daily
- pH:
- 6.33-7.41; determined twice weekly
- Dissolved oxygen:
- 44-85.8% of saturation; determined twice weekly
- Nominal and measured concentrations:
Measured: 57.78 +/- 31.85 ng/ml (equivalent to 57.78 ug/L)- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 40 cm x 25 cm x 20 cm, 15 L
- Aeration: dilution water tank was aerated with oil-free air; exposure tanks were not aerated
- Type of flow-through (e.g. peristaltic or proportional diluter): peristaltic
- Renewal rate of test solution (frequency/flow rate): 1.32 L/hr
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Madrid tap water, dechlorinated using sodium thiosulphate, purified with charcoal
- Nitrites: 0.003-0.070 mg NO2-
- Conductivity: 142.6-213 uS/cm
- Intervals of water quality measurement: every 3-4 days
OTHER TEST CONDITIONS
- Photoperiod: 12 hrs light/12 hrs dark
EFFECT PARAMETERS MEASURED: mortality, behaviour and toxic symptoms
TEST CONCENTRATIONS
- Test concentrations: acetone was used as a vehicle to obtain a testing concentration above the water solubility (37.2 +/- 11.7 ng/mL) of LAB. Note that all concentrations in the study are reported in units of ng/mL, which is equivalent to ug/L. The standard units for aquatic toxicity data are ug/L and so these units are used to report the results in this summary. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 57.78 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: behaviour, toxic symptoms, mortality
- Remarks on result:
- other: no toxic effects observed
- Duration:
- 21 d
- Dose descriptor:
- other: CL0
- Remarks on result:
- other: above water solubility limit
- Details on results:
- No mortality was observed in the test substance aquarium. One incidental non-treatment related mortality was observed in the control group. Another fish was removed from the control group due to attack by other fish. No toxic symptoms were observed in any of the test aquaria. Note that individual homologues were also tested. Phenyl C10, C12 and C18 also resulted in no toxic symptoms for the duration of the study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 21-day NOEC zebra fish exposed to LAB is 57.78 ug/L. The CL0 is above the water solubility limit.
- Executive summary:
In cases where no data were available on the target substance, Benzene, C15 -16 -alkyl derivs., data were read across from a structurally related material (the test substance).
Two groups of ten zebra fish were exposed in an extended acute test to a test solution of LAB for 21 days, where the LAB concentration was at or above the water solubility limit. This high concentration was obtained by using acetone as a vehicle. The measured test concentration was 57.78 ug/L, above the water solubility of 37.2 ug/L determined in a water solubility test conducted concurrently. No mortality, behavioural changes, or toxic symptoms were noted in the exposure groups. The 21 -day NOEC for zebra fish is 57.78 ug/L, the CL0 is above the water solubility limit of LAB.
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