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EC number: 212-855-9 | CAS number: 873-94-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 3,3,5-trimethylcyclohexan-1-one. Inoculumn concentration i.e, sludge concentration used was 30 mg/l while initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 3% by O2 consumption (BOD), 9% by TOC removal and 13% degradation by Test mat. analysis (GC) in 28 days. Thus, based on percentage degradation, the test substance 3,3,5-trimethylcyclohexan-1-one was considered to be not readily biodegradable in water.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Various studies for the test substance 3,3,5-trimethylcyclohexan-1-one(873-94-9) and its read across substances were reviewed to summarize the following information:
In an experimental study of target substance from J-check database, Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 3,3,5-trimethylcyclohexan-1-one. Inoculumn concentration i.e, sludge concentration used was 30 mg/l while initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 3% by O2 consumption (BOD), 9% by TOC removal and 13% degradation by Test mat. analysis (GC) in 28 days. Thus, based on percentage degradation, the test substance 3,3,5-trimethylcyclohexan-1-one was considered to be not readily biodegradable in water.
The other study for the target substance was predicted where Estimation Programs Interface Suite (EPI suite, 2017) was run to predict the biodegradation potential of the test compound 3,3,5-trimethylcyclohexan-1-one (CAS no.873 -94 -9) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 3,3,5-trimethylcyclohexan-1-one is expected to be not readily biodegradable in water.
Another experimental study for RA (CAS No.78-59-1) from J-check where Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance 3,5,5-trimethylcyclohex-2-enone. Inoculum concentration i.e, sludge concentration used was 30 mg/l while initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 1.5% by O2 consumption (BOD), 2.6% by TOC removal and 1% degradation by Test mat. analysis (GC) in 14 days. Thus, based on percentage degradation, the test substance 3,5,5-trimethylcyclohex-2-enone was considered to be not readily biodegradable in water.
Similarly in an experimental study from J-check for another RA (CAS No. 3452-97-9), Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance 3,5,5-trimethylhexan-1-ol. Inoculum concentration i.e, sludge concentration used was 30 mg/l while initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 4% by O2 consumption (BOD), 4% by TOC removal and 55% degradation by Test mat. analysis (GC) in 28 days. Thus, based on percentage degradation, the test substance 3,5,5-trimethylhexan-1-ol was considered to be not readily biodegradable in water.
Based on the data of target substance3,3,5-trimethylcyclohexan-1-one (873-94-9) and its read across chemicals 3,5,5-trimethylcyclohex-2-enone (78-59-1) and 3,5,5-trimethylhexan-1-ol (3452-97-9) both of which has 50-60% structural similarity it can be concluded that the test substance 3,3,5-trimethylcyclohexan-1-one is not expected to be readily biodegradable in water.
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