1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 November 2015 - 8 January 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted to current test guidelines in accordance with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate

EC Number:

411-700-4

EC Name:

1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate

Cas Number:

140921-24-0

Molecular formula:

C32H62N4O6

IUPAC Name:

2-[3-(heptan-3-yl)-1,2-oxazolidin-2-yl]ethyl N-{6-[({2-[3-(heptan-3-yl)-1,2-oxazolidin-2-yl]ethoxy}carbonyl)amino]hexyl}carbamate

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

Hardness (Ca+Mg) 0.24mmol/L (24 mg CaCO3/L)

Test temperature:

21-24°C

pH:

7.8

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

other: ErL50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: Loading rate

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

other: 72h-EyL50

Effect conc.:

30 mg/L

Nominal / measured:

nominal

Conc. based on:

other: Loading rate

Basis for effect:

other: yield

Any other information on results incl. tables

Under the conditions of the present study with Pseudokirchneriella subcapitata, 1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate inhibited both the growth rate and the yield of this fresh water algae species significantly at WAFs prepared at loading rates of 32 mg/L and higher.

The EL50 for growth rate inhibition (72h-E_RL₅₀) was beyond the tested concentration range, i.e. exceeded a WAF prepared at a loading rate of 100 mg/L.

The EL50 for yield inhibition (72h-E_YL₅₀), based on loading rate, was 32 mg/L with a 95% confidence interval ranging from 25 to 41 mg/L.

The 72h-NOEL for both growth rate and yield inhibition equalled a WAF prepared at a loading rate of 10 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EL50 for growth rate inhibition (72h-ERL50) was >100 mg/L

Executive summary:

The algal growth inhibition test was conducted according to the OECD Guideline 201 (Alga, Growth Inhibition Test). Due to the extremely low water solubility the test has been conducted by preparation of a Water Accomdated Fraction (WAF).

Under the conditions of the present study with Pseudokirchneriella subcapitata, 1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate inhibited both the growth rate and the yield of this fresh water algae species significantly at WAFs prepared at loading rates of 32 mg/L and higher.

The EL50 for growth rate inhibition (72h-E_RL₅₀) was beyond the tested concentration range, i.e. exceeded a WAF prepared at a loading rate of 100 mg/L.

The EL50 for yield inhibition (72h-E_YL₅₀), based on loading rate, was 32 mg/L with a 95% confidence interval ranging from 25 to 41 mg/L.

The 72h-NOEL for both growth rate and yield inhibition equalled a WAF prepared at a loading rate of 10 mg/L.