(Z)-3,7-dimethylocta-2,6-dienal

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given: comparable to guideline method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: BASF-test according to internal SOP

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Citral

EC Number:

226-394-6

EC Name:

Citral

Cas Number:

5392-40-5

IUPAC Name:

3,7-dimethylocta-2,6-dienal

Constituent 2

Reference substance name:

(Z)-3,7-dimethylocta-2,6-dienal

EC Number:

203-379-2

EC Name:

(Z)-3,7-dimethylocta-2,6-dienal

Cas Number:

106-26-3

Molecular formula:

C10H16O

IUPAC Name:

(2Z)-3,7-dimethylocta-2,6-dienal

Details on test material:

3,7-Dimethyloctadien-2,6-al-1 (Citral)
Purity: >= 95 %

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gassner

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

VEHICLE
0.5 % aqueous CMC preparation with 2-3 drops Cremophor EL as emulsifier

VOLUME APPLIED: 20 ml/kg (highest dose), 13.63 ml/kg (6810 mg/kg dose) and 10 ml/kg (three lowest doses)

Doses:

2150, 3160, 4640, 6810 and 10000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations (clinical signs): < 15, 15 and 30 min, 1, 2, 4 and 5 h, further on daily
- Frequency of weighing: at the beginning of the experiment, on day 3 and 7 and at the end of the experiment
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 6 800 mg/kg bw

Mortality:

see Table 1

Clinical signs:

other: 10000 mg/kg bw: the animals showed salivation, apathy, staggering, abdominal and lateral position, atony, narcotic state and no pain reflex before death. 6810 mg/kg bw: some animals showed apathy (1 h), staggering (1 h-5 h), salivation (15 min - 1 h), ruf

Gross pathology:

Dead animals: acute dilatation of the atriums; acute, congestive hyperemia; liver: peripheral lobule marking; intestine: atonic; thorax: sporadic hydrothorax;
Sacrificed animals: forestomach thickened, sporadic boutons, agglutinations with spleen and peritoneum

Any other information on results incl. tables

Pretest:

Mortality: 2/2 animals at 10,000 mg/kg bw, 1/2 animals at 4640 mg/kg bw, 0/2 animals at 1000, 215 or 46.4 mg/kg bw

Table 1: Mortality in main study

Dose (mg/kg bw)	No. of animals	Mortality within
		1h	24 h	48 h	7 d	14 d
10000	5M	0/5	4/5	5/5	5/5	5/5
	5F	0/5	4/5	5/5	5/5	5/5
6810	5M	0/5	0/5	2/5	2/5	2/5
	5F	0/5	0/5	1/5	2/5	2/5
4640	5M	0/5	0/5	0/5	0/5	0/5
	5F	0/5	0/5	0/5	0/5	0/5
3160	5M	0/5	0/5	0/5	0/5	0/5
	5F	0/5	0/5	0/5	0/5	0/5
2150	5M	0/5	0/5	0/5	0/5	0/5
	5F	0/5	0/5	0/5	0/5	0/5

Table 2: Mean body weight (male/female in g)

Dose (mg/kg bw)	0 d	3d	7d	14d
10000	220/160	-	-	-
6810	200/150	209/158	160/170	218/189
4640	200/160	210/168	209/173	224/187
3160	200/150	223/163	209/174	243/188
2150	170/150	205/170	227/145	211/188