Registration Dossier
Registration Dossier
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EC number: 268-610-1 | CAS number: 68131-13-5
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water: simulation testing on ultimate degradation in surface water
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 10- 12, 2009
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Naphthenic acids, reaction products with diethylenetriamine
- EC Number:
- 268-610-1
- EC Name:
- Naphthenic acids, reaction products with diethylenetriamine
- Cas Number:
- 68131-13-5
- Molecular formula:
- C16H21N3O2 (minimal)
- IUPAC Name:
- Naphthenic acids, reaction products with diethylenetriamine
- Details on test material:
- Test substance: MK195K
Stability: No particular stability concerns
Solubility: Insoluble
Odor: Aromatic
Storage: room temperature
Constituent 1
- Radiolabelling:
- no
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: seawater
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- % Degr.:
- 15
- Parameter:
- CO2 evolution
- Sampling time:
- 28 d
Any other information on results incl. tables
The COD for test item was determined to be 2.03 mg O2 per mg substance. The maximum percent biodegradation for MK195K of 15% was observed on day 28. The sodium acetate 2mg/L reference control achieved greater than 60% biodegradation within the 28 day test period. Dissolved oxygen depletion in the seawater controls remained within the test guideline of 30%; on day28 a maximum dissolved oxygen depletion of 5.6% occurred. Control oxygen level had decreased from 7.2 mg/L on day 0 to 6.8 mg/L on day 28.
Applicant's summary and conclusion
Validity criteria
- Validity criteria fulfilled:
- yes
- Conclusions:
- The maximum percent biodegradation for MK195K of 15% was observed on day 28.
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