Acetone oxime

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The analogue substance EAC3 undergoes rapid hydrolysis in aqueous to acetone oxime and the corresponding trisilanols. Trisilanols undergo continuous condensation reactions to produce higher molecular weight siloxanes which are considered biologically unavailable. Therefore, the observed toxicity is likely due to the acetone oxime and their values are comparable.

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

other: read-across

Title:

Unnamed

Year:

2013

Materials and methods

Principles of method if other than guideline:

Read-across approach from experimental results (test method according to OECD 423, GLP study) on the analogue substance EAC3.

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

58190-57-1

EC Number:

611-631-1

Cas Number:

58190-57-1

IUPAC Name:

58190-57-1

Constituent 2

Reference substance name:

2-propanone, 2,2'2,''-[O,O',O''-(ethylsilylidyne)trioxime]

IUPAC Name:

2-propanone, 2,2'2,''-[O,O',O''-(ethylsilylidyne)trioxime]

Details on test material:

- Name of test material (as cited in study report): EAC3
- Molecular formula (if other than submission substance): C11H23N3O3Si
- Molecular weight (if other than submission substance): 273.4
- Smiles notation (if other than submission substance): C\C(C)=N\O[Si](O\N=C(\C)C)(CC)O\N=C(/C)C
- InChl (if other than submission substance): 1/C11H23N3O3Si/c1-8-18(15-12-9(2)3,16-13-10(4)5)17-14-11(6)7/h8H2,1-7H3
- Structural formula attached as image file (if other than submission substance): see Fig.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 005.13 mg/kg bw

Based on:

test mat.

Remarks on result:

other: (read-across approach from an analogue)

Any other information on results incl. tables

As indicated in the European Chemical Agency Practical Guide 6 “How to report read –across and categories”, the structural grouping was realized using “OECD QSAR APPLICATION TOOL BOX” version 2.1.2. Presented results show that both substances have common (eco)toxicological behavior (attachment).

Table 1: Data Matrix, Analogue Approach:

CAS Number			Source chemical 58190-57-1	Target chemical 127-06-0
CHEMICAL NAME			EAC3 2-propanone, 2,2',2''-[O,O',O''-(ethylsilylidyne)trioxime]	Acetone oxime
PHYSICO-CHEMICAL DATA
Melting/Freezing point			Measured data: Freezing point: The substance did not solidify down to -22 ºC (EU Method A.1, OECD 102, GLP). Calculated data: Melting point: 14.10 ºC (EPI-Suite, MPBWIN v1.43).	Measured data (Handbook): 60 ºC
Boiling Point			Measured data: No boiling temperature could be determined since EAC3 decomposes before boiling (EU Method A.2, OECD 103, GLP). Calculated data: 301.81 ºC (EPI-Suite, MPBWIN v1.43).	Measured data (Handbook): 134.8 ºC
Relative density			Measured data: 1.011 at 20 ºC (EU Method A.3, OECD 109, GLP).	Measured data (Handbook): 0.9113 at 62 ºC
Vapour Pressure			Measured data: 0.0457 Pa at 25 ºC (EU Method A.4, OECD 104, GLP).	Calculated data (EPI-Suite, US EPA v4.1): 164 Pa at 25 ºC
Partition Coefficient (log Kow)			Measured data: An experimental determination was not possible since EAC3 is hydrolytically unstable (half-life < 12 h). Calculated data: Log Kow = 8.85 (EPI-Suite, KOWWIN v1.68).	Measured data (Jaros F et al., 2007): 0.20 Calculated data (EPI-Suite, US EPA v4.1): 1.2
Water solubility			Measured data: Not applicable since the substance is hydrolytically unstable (half-life < 12h). Calculated data: 0.0004829 mg/L at 25 ºC (EPI-Suite, WSKOWIN v1.41).	Measured data (Rosene MR et al., 1976): 334 g/L
ENVIRONMENTAL FATE and PATHWAY
Aerobic Biodegradation			Read-across from experimental results on analogues: Not ready biodegradable (read-across from Wasox-VMAC2) OECD 301B, GLP study. Not ready biodegradable (read-across from Wasox-MMAC2) OECD 301B, GLP study. Not ready biodegradable (read-across from acetone oxime) OECD 301D, GLP study. Calculated data (EPI-Suite, US EPA v4.1): Not ready biodegradable.	Experimental data: Not readily biodegradable OECD 301D, GLP study Calculated data (EPI-Suite, US EPA v4.1): Not readily biodegradable
ENVIRONMENTAL TOXICITY
Acute Toxicity to Fish			Read-across from the analogue acetone oxime: LC50 (96h) = 696.76 mg/L (Pimephales promelas) (Basis for effect: mortality) (Geiger et al. 1990; Method similar to OECD 203).	Experimental data: LC50 (96 h) = 558 mg/L (Pimephales promelas) (basis for effect: mortality) (Geiger et al. 1990; Method similar to OECD 203).
Acute Toxicity to Aquatic Invertebrates			Read-across from analogue Acetone oxime: EC50 (48h) = 678.73 mg/L (Daphnia magna) (Basis for effect: mobility) (OECD 202, GLP study)	Experimental data: EC50 (48h) = 544.34 mg/L (Daphnia magna) (basis for effect: mobility) (OECD 202, GLP study)
Toxicity to Aquatic Plants			Read-across from the analogue Acetone oxime: EC50 (72h) = 315.36 mg/L NOEC (72h) = 62.34 mg/L (Pseudokirchneriella subpicata) (Basis for effect: growth rate) (OECD 201, GLP study)	Experimental data: EC50 (72h) = 252.92 mg/L NOEC (72h) = 50 mg/L (Pseudokirchneriella subpicata) (Basis for effect: growth rate) (OECD 201, GLP study)
MAMMALIAN TOXICITY
Acute Toxicity: Oral			Experimental data: LD50 > 2500 mg/kg bw (rat, female) (OECD 423, GLP study).	Read-across from EAC3: LD50 > 2005.13 mg/kg bw (rat, female) (OECD 423, GLP study). Supporting data (Sax’s Handbook): LD0 > 500 mg/kg bw (rats).
Acute Toxicity: Inhalation			No data.	No data.
Acute Toxicity: Dermal			Read-across from acetone oxime: LD50 > 2493.77 mg/kg bw/day (rat, male/female) (OECD 402, GLP study)	Experimental data: LD50 > 2000 mg/kg bw/day (rat, male/female) (OECD 402, GLP study)
Skin irritation			Experimental data: Non-irritant. (OECD 439, GLP study)	Experimental data: According to CLP scoring system: Non-irritant (rabbits) (PDI, equivalent to OECD 404, GLP study)
Eye irritation			Experimental data: Non severe eye irritant. (OECD 438, GLP study) Non-irritant (rabbits). (OECD 405, GLP study)	Experimental data: According to CLP scoring system: Eye damage, Category 1 (rabbits) (Acute eye irritation, equivalent to OECD 405, GLP study)
Skin Sensitization			Read-across from Wasox-VMAC2: Not skin sensitizer (mice, female). (Local Lymph Node Assay, OECD 429, GLP study). Read-across from Wasox-MMAC2: Not skin sensitizer (mice, female). (Local Lymph Node Assay, OECD 429, GLP study).	Experimental data: Not skin sensitizer (mice, female) (Local Lymph Node Assay, OECD 429, GLP study).
Repeated Dose Toxicity			Read-across from Wasox-VMAC2: 28day-NOEL = 15.99 mg/kg bw/day (rats, male/female) (basis for effect: damage to peripheral erythrocytes without an impairment of the blood cell production in the bone marrow). (OECD 407, GLP study) Read-across from Wasox-MMAC2: 28day-NOEL = 16.54 mg/kg bw/day (rats, male/female) (basis for effect: damages to mature erythrocytes in the peripheral blood, with a parallel marked increase production of your erythrocytes by the presence of hematopoiesis in the spleen). (OECD 407, GLP study) Read-across from MIBKO: 90day-NOAEL = 11.87 mg/kg bw/day (rats, male/female) (effects on red blood cells, resulting in associated changes in the spleen) (OECD 408, GLP study)	Read-across from Wasox-VMAC2: 28day-NOEL = 12.82 mg/kg bw/day (rats, male/female) (basis for effect: damage to peripheral erythrocytes without an impairment of the blood cell production in the bone marrow). (OECD 407, GLP study) Read-across from Wasox-MMAC2: 28day-NOEL = 13.26 mg/kg bw/day (rats, male/female) (basis for effect: damages to mature erythrocytes in the peripheral blood, with a parallel marked increase production of your erythrocytes by the presence of hematopoiesis in the spleen). (OECD 407, GLP study) Read-across from MIBKO: 90day-NOAEL = 9.52 mg/kg bw/day (rats, male/female) (effects on red blood cells, resulting in associated changes in the spleen) (OECD 408, GLP study)
Genetic Toxicity in vitro		Gene mutation in bacteria	Read-across from acetone oxime: Negative with and without metabolic activation inSalmonella typhimuriumTA100, TA 97, TA 1535, and TA 98. (Equivalent to OECD 471, GLP study). Read-across from Wasox-VMAC2: Negative with and without metabolic activation inSalmonella typhimuriumTA97a, TA98, TA100, TA102 and TA1535. (OECD 471, GLP study) Read-across from Wasox-MMAC2: Negative with and without metabolic activation inSalmonella typhimuriumTA97a, TA98, TA100, TA102 and TA1535. (OECD 471, GLP study)   Supporting study: Negative with and without metabolic activation inSalmonella typhimuriumTA98, TA100, TA2637 andE. coliWP2 uvrA/pKM101. (Araki A et al., 1986)	Experimental data: Negative with and without metabolic activation inSalmonella typhimuriumTA100, TA 97, TA 1535, and TA 98. (Equivalent to OECD 471, GLP study).   Supporting study: Negative with and without metabolic activation inSalmonella typhimuriumTA98, TA100, TA2637 andE. coliWP2 uvrA/pKM101. (Similar to OECD 471, Araki A et al., 1986)
		Chromosomal aberration	Read-across from Wasox-VMAC2: Positive without metabolic activation with 20h treatment; negative with and without metabolic activation with 3 h treatment, in human lymphocytes. (OECD 473, GLP study) Read-across from Wasox-MMAC2: Negative with and without metabolic activation in human lymphocytes. (OECD 473, GLP study)	Read-across from Wasox-VMAC2: Positive without metabolic activation with 20h treatment; negative with and without metabolic activation with 3 h treatment, in human lymphocytes. (OECD 473, GLP study) Read-across from Wasox-MMAC2: Negative with and without metabolic activation in human lymphocytes. (OECD 473, GLP study)
		Mammalian gene mutation	Read-across from acetone oxime: Negative with and without metabolic activation at the TK +/- locus in L5178Y cells. (OECD 476, GLP study).	Experimental data: Negative with and without metabolic activation at the TK +/- locus in L5178Y cells. (OECD 476, GLP study).   Supporting studies: Not genotoxic in V79 chinese hamster cells as mutations to 6 -thioguanine (TG). (Similar to OECD 479, Haas-Jobelius M et al., 1991) Acetone oxime did not induce DNA repair synthesis (indicator of genotoxic) in rat hepatocytes (Haas-Jobelius et al. 1991), V79 cells (Haas-Jobelius et al. 1991; Andrae et al. 1999 and Kreis et al. 2000) and OSV cells (Kreis et al. 1998). (Similar to OECD 482).
Genetic Toxicity in vivo			Read-across from Wasox-VMAC2: Negative, the test substance did not produce relevant increases of the numbers of micronuclei in polychromatic erythrocytes. (Mammalian Erythrocyte Micronucleus Test, OECD Guideline 474, GLP Study).	Read-across from Wasox-VMAC2: Negative, the test substance did not produce relevant increases of the numbers of micronuclei in polychromatic erythrocytes. (Mammalian Erythrocyte Micronucleus Test, OECD Guideline 474, GLP Study).
Reproductive Toxicity	Toxicity to reproduction		Read-across from MIBKO: NOAEL (parental toxicity) = 23.74 mg/kg bw/day (male/female, rats) (based on histological effects on the spleen) NOAEL (P, reproductive toxicity) > 79.13 mg/kg bw/day (male/female, rats) (no effects observed at highest dose) NOAEL (F1 toxicity toxicity) > 79.13 mg/kg bw/day (male/female, rats) (no effects observed at highest dose) NOAEL (F1, developmental and sexual maturation) > 79.13 mg/kg bw/day (male/female, rats) (no effects observed at highest dose) (OECD 415, GLP).   Supporting study: Read-across from MIBKO: In the 90d RDT study, no effects were observed in the reproductive organs or tissues up to the highest dose tested. Read-across from Wasox-VMAC2: In the 28d RDT study, no effects were observed in the reproductive organs or tissues up to the highest dose tested Read-across from Wasox-MMAC2: In the 28d RDT study, no effects were observed in the reproductive organs or tissues up to the highest dose tested	Read-across from MIBKO: NOAEL (parental toxicity) = 19.04 mg/kg bw/day (male/female, rats) (based on histological effects on the spleen) NOAEL (P, reproductive toxicity) > 63.47 mg/kg bw/day (male/female, rats) (no effects observed at highest dose) NOAEL (F1 toxicity toxicity) > 63.47 mg/kg bw/day (male/female, rats) (no effects observed at highest dose) NOAEL (F1, developmental and sexual maturation) > 63.47 mg/kg bw/day (male/female, rats) (no effects observed at highest dose) (OECD 415, GLP).   Supporting study: Read-across from MIBKO: In the 90d RDT study, no effects were observed in the reproductive organs or tissues up to the highest dose tested. Read-across from Wasox-VMAC2: In the 28d RDT study, no effects were observed in the reproductive organs or tissues up to the highest dose tested Read-across from Wasox-MMAC2: In the 28d RDT study, no effects were observed in the reproductive organs or tissues up to the highest dose tested
	Developmental toxicity		No data.	No data.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Based on experimental data on the analogue EAC3 (test method according to OECD 423 and GLP), the read-across approach was applied and the acute oral LD50 of the test item acetone oxime was determined to be greater than 2005.13 mg/kg body weight infemale rats.

Executive summary:

An acute oral toxicity test was performed on analogue substance EAC3 according to OECD 423 (GLP study). Two groups of three female rats were treated by gavage with the test item at a dose level of 2500 mg/kg bw. No mortality was observed in the confirmatory group. All animals were clinical symptom (decreased activity, huncled back, prone position, incoordination, piloerection and creeping gait) free from three days after the treatment. No macroscopic observations were present at a dose level of 2500 mg/kg bw after 14 days of observation. The acute oral LD50 value of the the analogue EAC3 was found to be greater than 2500 mg/kg bw in female rats. Based on these results, the read-across approach was applied, and the acute oral LD50 for acetone oxime was determined to be > 2005.13 mg/kg bw in female rats.