Salicylaldehyde

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD test guideline study in compliance of GLP and with analytical measurements of the chemical.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

salicylaldehyde (CAS: 90-02-8, AGC SEIMI Chemical Co., Ltd., Lot. No.; 041208, Purity =99.8 %, Impurity = 0.2% (unclear))

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

4.8 mg/L

Nominal / measured:

meas. (geom. mean)

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

1.6 mg/L

Nominal / measured:

meas. (geom. mean)

Basis for effect:

other: area under growth curve

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.55 mg/L

Nominal / measured:

meas. (geom. mean)

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.55 mg/L

Nominal / measured:

meas. (geom. mean)

Basis for effect:

other: area under growth curve

Any other information on results incl. tables

- Measured Concentrations: Test concentrations were measured at the start and the end of test using by HPLC. Table1. Concentration of the Test Substance in Test Water. Nominal Conc. (mg/L) Measured Concentration (mg/L)(Percent of Nominal, %) Geometric　mean(mg/L) 0 hour (new) 72 hours (old) Control - - -- 1.0 0.81 (81) 0.37 (37) 0.55 2.2 1.74 (79) 0.83 (38) 1.20 4.8 3.56 (74) 1.97 (41) 2.65 10.6 8.47 (80) 5.36 (51) 6.74 23.4 19.66 (84) 13.49 (58) 16.29 - Water Chemistry: pH was measured for controls and each concentration. At the start and the end of test, the pH was 7.8 - 8.0 and 7.9 - 9.3, respectively. Temperature in algal culture cabinet was maintained at 23 +/- 2 C during test period. pH: 7.8 - 9.3,Temperature: 23.0 – 23.1 C. Table2. pH Values. Nominal Conc. (mg/L) pH 0hour 72hour Control 8 9.3 1.0 8 9 2.2 8 8.4 4.8 8 8 10.6 7.9 7.9 23.4 7.8 7.9 -Effect Data: Growth Rate Method EC50 (0 - 72 hr): 4.76 mg/L (mc), 95% Confidence Interval: 4.27 – 5.34 mg/L NOEC (0 - 72 hr): 0.55 mg/L (mc) mc: based on measured concentration -Effect Data: Area Method (area under growth curve) EC50 (0 - 72 hr): 1.57 mg/L (mc), 95% Confidence Interval: 1.43 – 1.72 mg/L NOEC (0 - 72 hr): 0.55 mg/L (mc) - Growth Inhibition (%) of Pseudokirchneriella subcapitata. Table3. Growth Rate, Inhibition and Cell density of Pseudokirchneriella subcapitata. Measured Conc. (mg/L) Growth Rate, Inhibition and Cell density Rate (Average) Inhibition (%) Cell density (cells/ml) u(0-72hr) Iu (0-72hr) (72hr) Control 1.33 - 546,000 0.55 1.33 0.23 540,333 1.20 1.14 14.8 302,333 2.65 0.88 34.2 139,000 6.74 0.60 55.1 61,267 16.29 0.15 88.6 16,133 - Growth Curves: Exponential growth phase during 72 hours. Measurement of algal biomass was not conducted in this test. Present values were obtained and estimated from growth rate based on number of algal cells.