Salicylaldehyde

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test was conducted according to OECD test guideline in accordance with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Salicylaldehyde

EC Number:

201-961-0

EC Name:

Salicylaldehyde

Cas Number:

90-02-8

Molecular formula:

C7H6O2

IUPAC Name:

2-hydroxybenzaldehyde

Details on test material:

Name of test material (as cited in study report): 2-hydroxybenzaldehyde　- Analytical purity:> 97.0 %　- Impurities (identity and concentrations): water, < 0.2 %　- Lot/batch No.: FGC01　- Stability under test conditions: IR chromatogrammes were measured before and after the experiment.- Storage condition of test material: Cooled place　

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

From 10 different places including sewage treatment plants, rivers, lake and inner sea in Japan, sludge and environmental water and soil were collected in September 1996. Then, these samples were mixed and cultivated with glucose, peptone, potassium dihydrogenphosphate.　Concentration of the sludge was 30 mg/L as suspended solid matters.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Details on study design:

6 bottles were prepared, which are as follows.　[Water + Test substance][Test medium + Test substance + Sludge] -1　[Test medium + Test substance + Sludge] -2　[Test medium + Test substance + Sludge] -3　[Test medium + Sludge]Volume of test medium or water: 300 mL　Volume of the test substance added was 30.3 mg which is injected with 29. 5 uL by micro-syringe.　30.3 mg = 29.5 uL x 1.022 g/cm3 (density)BOD was continuously measured by BOD meter over the cultivation period.　After the cultivation period, TOC was measured. Direct analysis by HPLC was also conducted after the cultivation period.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

other: BOD

Value:

2

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Degradability of 2-hydroxybenzaldehyde by BOD was 2 % according to OECD Test guideline 301C. However, 2-hydroxybenzaldehyde was not detected in the test system and whole amount of 2-hydroxybenzaldehyde was converted to the transformation product, 2-hydroxybenzoic acid (salicylic acid) after the cultivation period.