Benzyl(3-hydroxyphenacyl)methylammonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl(3-hydroxyphenacyl)methylammonium chloride

EC Number:

276-017-4

EC Name:

Benzyl(3-hydroxyphenacyl)methylammonium chloride

Cas Number:

71786-67-9

Molecular formula:

C16H17NO2.ClH

IUPAC Name:

benzyl(3-hydroxyphenacyl)methylammonium chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Benzyladrianon HCl
- Physical state: solid
- Analytical purity: 99.07 %
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge : Activated sludge freshly from the municipal sewage treatment plant : `Waterschap de Maaskant`,
`s Hertogenbosch, The Netherlands
- Storage conditions: continuous aeration
- Preparation of inoculum for exposure: Before use, the sludge was allowed to settel fo at least 30 min and the liquid decanted for use as inoculum at the amount of 10 mL/L of mineral medium.
- Concentration of sludge: 10 mL/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

18 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- pH: 7.5 -7.7
- Test temperature: 20.5 -22 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles with only inoculum
- Abiotic sterile control: 1 bottle with 40 mg/L sodium acetate + inoculum
- Toxicity control: 1 bottel with test item, refernce substance ( sodium acetate ) and inoculum

Reference substance

Reference substance:

other: sodium acetate

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0 - 0.5

Sampling time:

2 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

1.2 - 2.5

Sampling time:

9 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

4.6 - 9.6

Sampling time:

19 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

30.5 - 37.3

Sampling time:

29 d

BOD5 / COD results

Results with reference substance:

The reference substance was degraded at least 60% within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Benzyladrianon HCl was not readily biodegradable under the conditions of the modified Sturm test presently performed.

Executive summary:

Benzyladrianon HCl was tested for its ready biodegradability in the carbon dioxide evolution test ( modified Sturm test ) at 36 mg per 2 liters, corresponding to 12 mg TOC/L. The Theoretical carbon dioxide production of Benzyladrianon HCl was calculated to be 2.41 mg Carbon dioxid /mg.

The realtive degradation values calculated from the measurment during the test period revealed 37 % and 31 % degradation of Benzyladrianon HCl. Thus, the criterion for ready biodegradability was not met.