1-phenylethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of microorganism for the test chemical 1-phenylethan-1-ol :
1) To evaluate toxicity of test material on Staph. Aureus , E.coli , C.albicans
2)To evaluate antibacterial activity of test material on Activated sludge

Specific details on test material used for the study:

IUPAC name: 1-phenylethan-1-ol
Mol. formula: C8H10O
Molecular Weight: 122.166 g/mol
Substance form: Colourless liquid
Substance type: organic
InChI: 1S/C8H10O/c1-7(9)8-5-3-2-4-6-8/h2-7,9H,1H3
Smiles :c1(ccccc1)C(C)O

Test organisms (species):

other: 1) Staphylococcus aureus, Escherichia coli and Candida albicans 2) Activated sludge

Details on inoculum:

1) - Laboratory culture: Staphylococcus
aureus, Escherichia coli and Candida albicans, obtained from Monmouth Medical Center, Long Branch, New Jersey

- Method of cultivation: Stock cultures were maintained on agar slants composed of tryptone , 0.3%; yeast extract, 0.3%; glucose, 09 K2HPO 4, 0.1%, and agar, 1% (referred to as TGY agar).

- Pretreatment: Test chemicals were tested initially at 0.1, 1.0 and 10
mg/l to determine the approximate range of activity. When no positive materials were found, these were repeated at 10, I00, and 1000 mg/l

Test type:

static

Water media type:

other: 1. frreshwater 2. Growth media TGY broth

Total exposure duration:

24 h

Test temperature:

37°C

Details on test conditions:

1) - Test vessel: 18 x 150 mm tubes; 10.0 ml/tube).
Fill volume : Test materials was added to the tubes as 10% (w/v) solutions.
-Tubes were inoculated with 50/ml of a 1 : 10 dilution in sterile 0.85% saline of a 24-hour shake culture (TGY broth). A minimum of 300,000 viable organisms were added to each tube. Tubes were mixed incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity. on a Vortex mixer and incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity

Duration:

24 h

Dose descriptor:

other: MIC

Effect conc.:

> 1 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

inhibition of respiration due to nitrification

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical 1-phenylethan-1 ol is not likely to be toxic to microorganism atleast in the concentration range of 1000 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals benzenemethanol and benzoic acid

has been reviewed to determine the short term toxicity of fish of the test chemical 1-phenylethan-1 ol (98 -85 -1).The studies are as mentioned below:

1.The minimum inhibition concentration of test material benzenemethanol was evaluated for its toxicity on Staph. Aureus , E.coli , C.albicans .

Staphylococcus aureus, Escherichia coli and Candida albicans, obtained from Monmouth Medical Center, Long Branch, New Jersey. Stock cultures were maintained on agar slants composed of tryptone , 0.3%; yeast extract, 0.3%; glucose, 0.9 K2HPO 4, 0.1%, and agar, 1% (referred to as TGY agar). Test chemicals were tested initially at 0.1, 1.0 and 10 mg/l to determine the approximate range of activity. When no positive materials were found, these were repeated at 10, I00, and 1000 mg/l.18 x 150 mm tubes; 10.0 ml/tube was used as a test vessel .Test materials was added to the tubes as 10% (w/v) solutions. Tubes were inoculated with 50/ml of a 1 : 10 dilution in sterile 0.85% saline of a 24-hour shake culture (TGY broth). A minimum of 300,000 viable organisms were added to each tube. Tubes were mixed incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity. on a Vortex mixer and incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity.The minimum inhibition concentration of test chemical Benzenemethanol on Staph. Aureus , E.coli , C.albicans was observed to be >1000 mg/l

 

2.Test material benzoic acid was used as a test material to evaluate toxicity to microorganisms as per OECD guideline 209.Activated sludge was used as the test culture at pH 7.5 for 3 hr.The EC50 value on the basis of respiration inhibition test was observed to be >1000 mg/l.

Description of key information

Toxicity to microorganism:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 1-phenylethan-1 ol (98 -85 -1).The studies are as mentioned below:

1.The minimum inhibition concentration of test material was evaluated for its toxicity on Staph. Aureus , E.coli , C.albicans .

Staphylococcus aureus, Escherichia coli and Candida albicans, obtained from Monmouth Medical Center, Long Branch, New Jersey. Stock cultures were maintained on agar slants composed of tryptone , 0.3%; yeast extract, 0.3%; glucose, 0.9 K2HPO 4, 0.1%, and agar, 1% (referred to as TGY agar). Test chemicals were tested initially at 0.1, 1.0 and 10 mg/l to determine the approximate range of activity. When no positive materials were found, these were repeated at 10, I00, and 1000 mg/l.18 x 150 mm tubes; 10.0 ml/tube was used as a test vessel .Test materials was added to the tubes as 10% (w/v) solutions. Tubes were inoculated with 50/ml of a 1 : 10 dilution in sterile 0.85% saline of a 24-hour shake culture (TGY broth). A minimum of 300,000 viable organisms were added to each tube. Tubes were mixed incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity. on a Vortex mixer and incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity.The minimum inhibition concentration of test chemical on Staph. Aureus , E.coli , C.albicans was observed to be >1000 mg/l.

2. Test material was used as a test material to evaluate toxicity to microorganisms as per OECD guideline 209.Activated sludge was used as the test culture at pH 7.5 for 3 hr.The EC50 value on the basis of respiration inhibition test was observed to be >1000 mg/l.

 Based on the above effect concentration it can be concluded that test substance 1-phenylethan-1 ol has no adverse effect on microorganism

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

Toxicity to microorganism:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 1-phenylethan-1 ol (98 -85 -1).The studies are as mentioned below:

1.

The minimum inhibition concentration of test material Benzenemethanol was evaluated for its toxicity on Staph. Aureus , E.coli , C.albicans .

Staphylococcus aureus, Escherichia coli and Candida albicans, obtained from Monmouth Medical Center, Long Branch, New Jersey. Stock cultures were maintained on agar slants composed of tryptone , 0.3%; yeast extract, 0.3%; glucose, 0.9 K2HPO 4, 0.1%, and agar, 1% (referred to as TGY agar). Test chemicals were tested initially at 0.1, 1.0 and 10 mg/l to determine the approximate range of activity. When no positive materials were found, these were repeated at 10, I00, and 1000 mg/l.18 x 150 mm tubes; 10.0 ml/tube was used as a test vessel .Test materials was added to the tubes as 10% (w/v) solutions. Tubes were inoculated with 50/ml of a 1 : 10 dilution in sterile 0.85% saline of a 24-hour shake culture (TGY broth). A minimum of 300,000 viable organisms were added to each tube. Tubes were mixed incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity. on a Vortex mixer and incubated at 37°C for 18 to 24 hr, sufficient time to obtain significant turbidity.The minimum inhibition concentration of test chemical Benzenemethanol on Staph. Aureus , E.coli , C.albicans was observed to be >1000 mg/l

 

2. Test material was used as a test material to evaluate toxicity to microorganisms as per OECD guideline 209.Activated sludge was used as the test culture at pH 7.5 for 3 hr.The EC50 value on the basis of respiration inhibition test was observed to be >1000 mg/l.

 Based on the above effect concentration it can be concluded that test substance 1-phenylethan-1 ol has no adverse effect on microorganism