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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Instead of reference substance 3,5-Dichlorophenol potassiumdichromat was used. EC- analysis was done through graphical way. GLP was done.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Potassiumdichromate instead of 3,4-Dichlorophenol
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5-dimethoxyaniline
EC Number:
203-040-9
EC Name:
2,5-dimethoxyaniline
Cas Number:
102-56-7
Molecular formula:
C8H11NO2
IUPAC Name:
2,5-dimethoxyaniline
Details on test material:
purity 99.9 %

Test solutions

Vehicle:
no
Details on test solutions:
pre-tests: 10 and 100 mg/l
tests concentration (mg/l): 58, 100, 180, 320, 580 and 1000, factor 1.8
test concentraion for reference substance mg/l: 32, 58, 100

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
pre-treatment: after sedimentation washed with dechlorinated tap water two times.
concentraion 1.781 g/l

Study design

Test type:
static
Water media type:
brackish water
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
18 - 22 °C
Dissolved oxygen:
O2-detection continously with O2-Electrode (polarographical) and voltage recorder
Details on test conditions:
concentration of reference substance: 32, 58 and 100 mg/l
growth inhibition of reference substance: 58 mg/l: 51 %
Reference substance (positive control):
yes
Remarks:
potassium dichromate (Fa. Merck, Germany)

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
< 58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: not detectable
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: not detectable
Duration:
3 h
Dose descriptor:
EC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: not detectable
Results with reference substance (positive control):
Potassiumdichromat was tested with 32, 58 and 100 mg/l. At 58 mg/l 51 % growth inhibition was found.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 is > 1000 mg/l
Executive summary:

EC50 is > 1000 mg/l