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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study report which meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: company guideline similar to OECD 401
Principles of method if other than guideline:
Only females were tested. The LD50 was determined according to the method of Kärber.
GLP compliance:
no
Remarks:
prior to GLP implementation
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5-dimethoxyaniline
EC Number:
203-040-9
EC Name:
2,5-dimethoxyaniline
Cas Number:
102-56-7
Molecular formula:
C8H11NO2
IUPAC Name:
2,5-dimethoxyaniline
Details on test material:
- Name of test material (as cited in study report): Aminohydrochinondimethyläther

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS

- Weight at study initiation: 90 -116 g
- Fasting period before study: 16 h
- Diet (e.g. ad libitum): standard feed (ALTROMIN R), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum


Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: mucous starch
Details on oral exposure:
- Concentration in vehicle: 4%
- Route: oral, gavage
Doses:
250, 400, 630, 1000 mg/kg bw
No. of animals per sex per dose:
10
Control animals:
no

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
578 mg/kg bw
Based on:
test mat.
Mortality:
250 mg/kg bw: 0/10
400 mg/kg bw: 3/10
630 mg/kg bw: 6/10
1000 mg/kg bw: 10/10

The animals died within 1- 96 hours after application.
Clinical signs:
other: Animals that died showed disequilibrium, lateral or prone position, narcosis-like state and physical poor health.
Gross pathology:
Cross pathology of animals found dead didn't reveal any abnormal findings.

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
LD50: 578 mg/kg bw.
Executive summary:

Acute toxicity of Aminohydrochinondimethyläther was tested according to a company guideline reasonably similar to OECD 401. Following this protocol doses of 250, 400, 630, 1000 mg/kg bw were administered by gavage to 10 female rats per dose.

During the observation period of 14 days mortality occured at doses of 400 mg/kg bw (3/10), 630 mg/kg bw (6/10), and 1000 mg/kg bw (10/10). LD50 was calculated to be 578 mg/kg bw.

Animals that died showed disequilibrium, lateral or prone position, narcosis-like state and physical poor health. Cross pathology of animals found dead didn't reveal any abnormal findings.