N,N'-(2-(4-(2-aminobenzamido)butyl)pentane-1,5-diyl)bis(2-aminobenzamide)

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental Phase: 14 June 2020 to 05 September 2020.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Details on study design: HPLC method:

The sample, dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:

HPLC System : Agilent Technologies 1100 Series, incorporating
workstation and autosampler
Detector type : variable wavelength (VW)
Column : XSelect Cyano 5µm (150 x 4.6 mm id)
Column temperature : 30 °C
Mobile phase : tetrahydrofuran: purified water (40:60 v/v)
pH of mobile phase : 7.2
Flow-rate : 1.0 mL/min
Injection volume : 10 µL
VW detector wavelength : 210 nm (dead time and reference standards)
253 nm (sample)

Key result

Sample No.:

#1

Type:

log Koc

Value:

ca. 3.24 dimensionless

Remarks on result:

other: HPLC column temperature was 30°C. The test was performed at approximately neutral pH.

Key result

Sample No.:

#2

Type:

log Koc

Value:

ca. 3.24 dimensionless

Remarks on result:

other: HPLC column temperature was 30°C. The test was performed at approximately neutral pH.

 

Results

The retention times, capacity factor and log10 K_oc value determined for the sample are shown in the following table:

 

 

Injection	Retention Time (minutes)	Capacity Factor (k')	Log10 k'	Log10 Koc
1	9.213	4.3258	0.63607	3.34
2	9.218	4.3287	0.63635	3.24

 

Mean log₁₀ K_oc: 3.24

Adsorption coefficient: 1.72 x 10³

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test item has been determined to be 1.72 x 10^3, log10 Koc 3.24. The result classifies the test item as having low mobility.

Executive summary:

Introduction

The Adsorption/Desorption coefficient of the test item was undertaken using a HPLC screening method, designed to be compatible with the following guidelines:

 

• Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008


• Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001. Method 7  

 

Method

The test system utilised a high-performance liquid chromatograph and a commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.   

 

Results

The retention times, capacity factor and log10 K_oc value determined for the sample are shown in the following table:

 

 

Injection	Retention Time (minutes)	Capacity Factor (k')	Log10 k'	Log10 Koc
1	9.213	4.3258	0.63607	3.34
2	9.218	4.3287	0.63635	3.24

 

 

Conclusion

The adsorption coefficient of the test item has been determined to be 1.72 x 10³, log10 K_oc 3.24. The result classifies the test item as having low mobility.

Description of key information

Adsorption/ Desorption data summary

Key value for chemical safety assessment

Koc at 20 °C:

1 720

Additional information

The adsorption coefficient of the test item has been determined to be 1.72 x 10^3, log10 Koc 3.24. The result classifies the test item as having low mobility.