Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of (2S-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2R,4R) and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2S,4S) and (2R-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran
EC Number:
939-429-1
Cas Number:
16409-43-1
Molecular formula:
C10H18O
IUPAC Name:
Reaction mass of (2S-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2R,4R) and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2S,4S) and (2R-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran
Details on test material:
- Name of test material (as cited in study report): Rose Oxide 90/10
- Substance type: chemical
- Physical state: liquid, colorless
- Analytical purity: 99.1 %, as a mixture of two isomers
- Isomers composition: cis-trans isomers
- Lot/batch No.: R:7694
- Stability under test conditions:
- Storage condition of test material: stored in a refrigerator
- Water solubility: 64 mg/L

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At the start of the exposure (0 h) samples from vessels without daphnids and at the end of
the exposure (48 h) samples from vessels with daphnids were analysed.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test concentrations were prepared separately for each test vessel (differential loading).
The test substance was homogenized by shaking of the container before test substance was
removed. The test substance was weighed into the volumetric flask and a defined volume of
test medium was added. The test concentrations were stirred (magnetic stir plate) for about 1
hour at 20 ± 2 °C to ensure that the test substance was completely dissolved.The test was
performed in a closed system. All test solutions appeared colourless and clear.
- Controls: media control
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no

CULTURE CONDITIONS
- Acclimation conditions (same as test or not): as test
- Type and amount of food: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not indicated

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
total hardness: 2.20 - 3.20 mmol/L
Test temperature:
20.4 - 21.0 °C
pH:
7.9 - 8.0
Dissolved oxygen:
8.1 - 9.2
Nominal and measured concentrations:
nominal: 0, 5.0, 10, 22, 50, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes (glass) sealed with gas impermeable teflon caps. (nominal volume 23 mL)
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, approx. 23 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.22 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 202
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-night regime
- Light intensity: 20 – 850 Lux at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility after 0, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx 2

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
33.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
48 h EC0 22 mg/L (nominal)
48 h EC100 50 mg/L (nominal)

Applicant's summary and conclusion