Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of (2S-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2R,4R) and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2S,4S) and (2R-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran
EC Number:
939-429-1
Cas Number:
16409-43-1
Molecular formula:
C10H18O
IUPAC Name:
Reaction mass of (2S-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2R,4R) and Tetrahydro-4-methyl-2-(2-methylpropen-1-yl)pyran (2S,4S) and (2R-cis)-tetrahydro-4-methyl-2-(2-methyl-1-propenyl)-2H-pyran
Details on test material:
- Name of test material (as cited in study report): Rose Oxide 90/10
- Substance type: chemical
- Physical state: liquid
- Analytical purity: 99.1 % as a mixture of two isomers
- Lot/batch No.: R:7694
- Water solubility: 64 mg/L

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 8 ml of the synthetic medium were added to the test vessels with test substance preparations, which were prepared the previous day.
Aliquots of the stock solutuon with reference substance were pipetted to the test vessels and made with demineralised water to a volume 180 mL. After that 8 mL syntehtic medium were pipetted in each test vessel with reference substance.
To prepare the blanc control assays 180 mL of demineralised water and 8 mL synthetic medium were mixed.
12 mL deminderalised water were added to each test vessel. After addition of 50 mL of the inoculum suspension (dry weight: 7.5 g/L) the incubation was started by aeration of the test vessels with pressure air. The vessels for the blanc control assays were prepared according to the same procedure without addition of test- or reference substance.

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Source: Activated sludge from the municipal wastewater treatment plant of Mannheim/Baden Württemberg, Germany was collected on 19 Dec 2007 from the aeration tank of the plant

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min

Test conditions

Test temperature:
22 +/- 2 °C
pH:
7.2 - 7.9
Dissolved oxygen:
> 6.5 mg/L
Nominal and measured concentrations:
nominal: 0, 62.4, 125, 250, 500, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erylenmeyer vessel, nominal 250 ml
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
- Biomass loading rate: 50 ml of 7.5 g DS/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209
- Intervals of water quality measurement: at test start and end

OTHER TEST CONDITIONS
- Adjustment of pH: no

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion