Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study according to GLP and OECD guidline
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge Zentralklarwerks der Stadtentsorgung
Neustadt (ESN), Im Altenschemel, NW-Lachen-Speyerdorf, lot 19022001

- Storage conditions: aerobic
- Storage length: 2 days
- Pretreatment: 1 hour sedimentation
- Water filtered: no
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
39.8 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 21 +- 2°C
- CEC (meq/100 g):
- Continuous darkness: yes/no
- Other:


TEST SYSTEM
- Culturing apparatus: narrow mouth bottle
- Measuring equipment: TOC 5050-A der Fa. Shimadzu
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes



CONTROL AND BLANK SYSTEM
- Abiotic sterile control: 10 ml 1% HgCl2
- Toxicity control: Test substance + aniline


Reference substance
Reference substance:
aniline

Results and discussion

Preliminary study:
not performed
Test performance:
criteria for test validation fulfilled
% Degradation
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Details on results:
no biodegradation observed

BOD5 / COD results

Results with reference substance:
biodegradation 95% after 5 days

Any other information on results incl. tables

no overall remarks

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
under test conditions no biodegradation observed
Executive summary:

Thiothiamin was not readily biodegradable under the conditions of the OECD test 301E.