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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
EC Number:
302-446-4
EC Name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
Cas Number:
94109-09-8
Molecular formula:
C48H84O6
IUPAC Name:
tri(tridecyl) benzene-1,2,4-tricarboxylate
Test material form:
liquid
Details on test material:
- Moisture content: <= 0.1%
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot P-2960F-10
- Expiration date of the lot/batch: June 29, 2013
- Purity test date: > 99%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: Stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: WAF (wateraccomodated fractions) due to insolubility of the test material
- Eluate: water
-- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): To prepare the WAF, 0.1001 g of the test item was weighted onto a watch glass. Dilution water (approximately 100 mL) was used to rinse the test item off the watch glasss into a 2 L beaker which contaied approximately 600 mL of dilution water. The total volume was brought to 1 L in the beaker using dilution water. A WAF control was prepared in teh sme way with the xeception that no test item was added to the 1 L of dilution water.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchneriella subcapita
- Strain: CC Strain 37
- Source (laboratory, culture collection): obtained from the Canadian Phycological Culture Centre, Waterloo, ON , Canada, as a sterile liquid starter culture.
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: The algae were cultured in 250 mL glass Erlenmeyer flasks, using Nutrient Medium and was based on the procedures in the Test Method OECD 201: Alga, Growth Inhibition (DCN80-C-017)

ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): see the report
- Any deformed or abnormal cells observed: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
23°C
pH:
7.4 to 7.5 (initial) - 7.9 to 8.0 (final)
Conductivity:
92 to 94 (initial) microS/cm
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open / closed : closed
- Material, size, headspace, fill volume: 50 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 1.02 X 104 cells/mL
- Control end cells density:
- No. of organisms per vessel:6
- No. of vessels per concentration (replicates):6
- No. of vessels per control (replicates):6
- No. of vessels per vehicle control (replicates):6

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: WAF
- Conductivity: 92 to 94 at 0h microS/cm
- Culture medium different from test medium: none

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod:24h light
- Light intensity and quality: 6461-8241 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] : counting chamber
- Other: Conductivity and pH

TEST CONCENTRATIONS
- Spacing factor for test concentrations: WAF 100 mg/L
- Justification for using less concentrations than requested by guideline: insolubility of the test item
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the nominal concentrations of the test material, using both Specific Growth Rate and Cell Yield, the estimated 72h IC50, IC25 and the LOEC (Lowest Observed Effect Concentration) were > 100 mg/L.
The NOEC (No Observed Effect Concentration) was 100 mg/L.