Registration Dossier
Registration Dossier
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EC number: 295-893-9 | CAS number: 92129-93-6
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Whey, lactose-low
- EC Number:
- 295-893-9
- EC Name:
- Whey, lactose-low
- Cas Number:
- 92129-93-6
- IUPAC Name:
- Whey, lactose-low
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 6 to 24 hours old
Study design
- Test type:
- not specified
- Water media type:
- other: reverse osmosis water
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 +/- 25 mg calcium carbonate/L
- Test temperature:
- 21°C
- pH:
- 7.8 +/- 0.2
- Dissolved oxygen:
- The water was aerated to an initial oxygen saturation of > 90%.
The dissolved oxygen concentration at the end of the test were 60% of the air saturation value at the temperature used in all dishes. - Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- 0, 12.5, 25, 50 and 100 mg/L (nominal concentrations)
- Details on test conditions:
- Two replicates were used for each test concentration, including the controls (dilution water), with ten animals in each.
Light rythm of 16 hours light: 8 hours darkness. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- no positive control
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is not considered to be toxic to aquatic organisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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