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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Whey, lactose-low
EC Number:
295-893-9
EC Name:
Whey, lactose-low
Cas Number:
92129-93-6
IUPAC Name:
Whey, lactose-low

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
6 to 24 hours old

Study design

Test type:
not specified
Water media type:
other: reverse osmosis water
Total exposure duration:
48 h

Test conditions

Hardness:
250 +/- 25 mg calcium carbonate/L
Test temperature:
21°C
pH:
7.8 +/- 0.2
Dissolved oxygen:
The water was aerated to an initial oxygen saturation of > 90%.
The dissolved oxygen concentration at the end of the test were 60% of the air saturation value at the temperature used in all dishes.
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
0, 12.5, 25, 50 and 100 mg/L (nominal concentrations)
Details on test conditions:
Two replicates were used for each test concentration, including the controls (dilution water), with ten animals in each.
Light rythm of 16 hours light: 8 hours darkness.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
no positive control

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance is not considered to be toxic to aquatic organisms.