Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

Neurotoxicity

Administrative data

Endpoint:

neurotoxicity, other

Remarks:

acute oral and intraperitoneal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: Assessment of physiological neurotoxicity signs after administration of test substance
- Short description of test conditions: test substance was administered once orally or intraperitoneally and the animals were observed for 28 d.
- Parameters observed: neurotoxic symptoms such as ataxy or paralysis

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

EC Number:

220-482-8

EC Name:

Diethyl bis(2-hydroxyethyl)aminomethylphosphonate

Cas Number:

2781-11-5

Molecular formula:

C9H22NO5P

IUPAC Name:

diethyl {[bis(2-hydroxyethyl)amino]methyl}phosphonate

Test material form:

liquid

Test animals

Species:

hen

Strain:

other: Leghorn (white)

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Breeder Gierlich, Bochum, Germany

Administration / exposure

Route of administration:

other: oral and intraperitioneal

Vehicle:

water

Remarks:

destilled

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

After a single treatment, animals were observed for 28 days.

Frequency of treatment:

Once

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

500 mg/kg bw/day

Remarks:

oral

Doses / concentrations 2

Dose / conc.:

1 000 mg/kg bw/day

Remarks:

oral

Doses / concentrations 3

Dose / conc.:

2 500 mg/kg bw/day

Remarks:

oral and intraperitoneal

Doses / concentrations 4

Dose / conc.:

3 750 mg/kg bw/day

Remarks:

intraperitoneal

Doses / concentrations 5

Dose / conc.:

5 000 mg/kg bw/day

Remarks:

oral and intraperitoneal

No. of animals per sex per dose:

one animal/dose (for 500, 1000 and 2500 mg/kg bw);
5 animals/dose (for 5000 mg/kg bw)

Control animals:

no

Examinations

Observations and clinical examinations performed and frequency:

CAGE SIDE OBSERVATIONS: Yes
- Effects observed: neurotoxic symptoms (e.g. ataxia, paralysis)

DETAILED CLINICAL OBSERVATIONS: No

BODY WEIGHT: No

WATER CONSUMPTION AND COMPOUND INTAKE: No data

OPHTHALMOSCOPIC EXAMINATION: No

Specific biochemical examinations:

NEUROPATHY TARGET ESTERASE (NTE) ACTIVITY: No

CHOLINESTERASE ACTIVITY: No

Neurobehavioural examinations performed and frequency:

FUNCTIONAL OBSERVATIONAL BATTERY: No

LOCOMOTOR ACTIVITY: No

AUDITORY STARTLE REFLEX HABITUATION: No

LEARNING AND MEMORY TESTING: No

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Body weight and weight changes:

not examined

Food consumption and compound intake (if feeding study):

not examined

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

not examined

Clinical biochemistry findings:

not examined

Urinalysis findings:

not examined

Behaviour (functional findings):

not examined

Immunological findings:

not examined

Organ weight findings including organ / body weight ratios:

not examined

Gross pathological findings:

not examined

Neuropathological findings:

not examined

Histopathological findings: non-neoplastic:

not examined

Histopathological findings: neoplastic:

not examined

Effect levels

Key result

Dose descriptor:

dose level:

Effect level:

> 5 000 mg/kg bw/day

Based on:

test mat.

Sex:

not specified

Basis for effect level:

behaviour (functional findings)

Applicant's summary and conclusion

Executive summary:

No neurotoxic symthomes were reported in this early acute delayed neurotoxicity study in chickens.