2,2'-(m-tolylimino)diethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 November 1999 - 26 November 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control and 100 mg/L
- Sampling method: at t=0, 24, 48, 72 and 96 h

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test item was directly added to the test medium at a concentration of 100 mg/L. The solution was stirred on a magnetic stirrer for 2 hours before the start of the test.
- Controls: yes, medium without additives was used as a conrol.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Strain: Hamilton Buchanan
- Source: Bio International B.V. (The Netherlands)
- Age at study initiation (mean and range, SD): approx. 6 months old
- Length at delivery: 2.5 - 3.5 cm
- Delivery date: 1999-09-09
- Mortality: <2% mortality was observed during the period between delivery of the fish and the experimental start date.
- Acclimation conditions: synthetic freshwater according to ISO


FEEDING DURING TEST : not indicated

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

244.52 CaCO3/L (13.7 deutscher Härtegrad)

Test temperature:

Control and 100 mg/L: 21.1 - 21.9 °C throughout the test

pH:

Control and 100 mg/L: 7.6-7.9 throughout the test

Dissolved oxygen:

Control: 8.5 - 8.9 mg O2/L throughout the test
100 mg/L: 8.6 - 8.9 mg O2/L throughout the test

Nominal and measured concentrations:

Nominal concentration: 100 mg/L
Measured concentrations: 71, 68, 68, 68 and 68 mg/L at t=0, 24, 48, 72 and 96 h, respectively.

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria with a size of 300 x 135 x 200 mm, fill volume: 5 L
- Aeration: yes
- No. of organisms: 10 for the test solution and 10 for the control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to ISO

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark

EFFECT PARAMETERS MEASURED: mortality at t=2, 24, 48, 72 and 96 h

RANGE-FINDING STUDY: no range-finding study was conducted

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 68.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: Based on TOC measurements

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 102 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

Calculated test item concentration from TOC measurements

Basis for effect:

mortality (fish)

Details on results:

- No mortality was observed in control and at the tested concentration of 100 mg/L
- No behavioural abnormalities were observed throughout the test for both control and test item concentration 100 mg/L
- The report indicated a 96h-LC0 of > 102.9 mg/L. However, based on the measured concentrations, an arithmetic mean exposure concentration of 68.6 mg/L was calculated by the applicant.
- Length at study end: 2.7 - 3.5 cm (control and 100 mg/L)
- Weight at study end, average of 10 fish: 0.364 g (control) and 0.325 g (100 mg/L)

Validity criteria fulfilled:

yes

Remarks:

See 'Overall Remarks'

Conclusions:

The 96h-LC50 of N,N-Bis-(hydroxyethyl)-m-toluidin was determined in a limit test according to EU method C.1 to exceed the tested concentration (i.e. >68.6 mg/L, mean measured exposure concentration based on TOC measurement).

Executive summary:

An acute fish toxicity test was performed according to EU method C.1. A concentration of 100 mg/L of the test item was directly applied to the test medium, the test solution was stirred for 2 hours on a magnetic stirrer and this solution was used as the test solution. Ten zebrafish were exposed to the limit concentration and a control for 96 hours. Observations for mortality were made at t=2, 24, 48, 72 and 96 h. Samples taken from the test solution and the control at t=0, 24, 48, 72 and 96 h were analysed by TOC analysis. Measured concentrations were 71 -68 mg/L. During the exposure period, no mortality occurred and no abnormalities were observed. Therefore, the LC50 was determined to exceed the limit concentration tested, i.e. >68.6 mg/L based on the mean measured exposure TOC concentration, corresponding to >102 mg/L test item.

Description of key information

In a limit study according to EU method C.1, the 96h-LC50 for freshwater fish was determined to be >102 mg/L based on mean measured concentration.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

102 mg/L

Additional information

An acute fish toxicity test was performed according to EU method C.1. A concentration of 100 mg/L of the test item was directly applied to the test medium, the test solution was stirred for 2 hours on a magnetic stirrer and this solution was used as the test solution. Ten zebrafish were exposed to the limit concentration and a control for 96 hours. Observations for mortality were made at t=2, 24, 48, 72 and 96 h. Samples taken from the test solution and the control at t=0, 24, 48, 72 and 96 h were analysed by TOC analysis. Measured concentrations were 71 -68 mg/L. During the exposure period, no mortality occurred and no abnormalities were observed. Therefore, the LC50 was determined to exceed the limit concentration tested, i.e. >68.6 mg/L based on the mean measured exposure TOC concentration, corresponding to >102 mg/L test item.