Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-067-3 | CAS number: 6107-56-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 May 2018 to 04 June 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Version / remarks:
- adopted 28 July 2015
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.46 (In Vitro Skin Irritation: Reconstructed Human Epidermis Model Test)
- Version / remarks:
- Amended by EC No. 640/2012 OJ No. L193, 20 July 2012.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Calcium di(octanoate)
- EC Number:
- 228-067-3
- EC Name:
- Calcium di(octanoate)
- Cas Number:
- 6107-56-8
- Molecular formula:
- C8H16O2.1/2Ca
- IUPAC Name:
- calcium di(octanoate)
- Test material form:
- solid
- Details on test material:
- Purity: 100 %
Constituent 1
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- other: Adult donors
- Justification for test system used:
- In the interest of sound science and animal welfare, a sequential testing strategy is recommended to minimize the need of in vivo testing. One of the validated in vitro skin irritation tests is the EPISKIN test, which is recommended in international guidelines (e.g. OECD and EC).
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE
- Model used: EPISKIN Small ModelTM (EPISKIN-SMTM, 0.38 cm2)
- Tissue batch number(s): 18-EKIN-022
- Production date: 29 May 2018 (Cerification and release date)
- Shipping date: Not reported
- Delivery date: Not reported
- Date of initiation of testing: 28 May 2018
TEMPERATURE USED FOR TEST SYSTEM
- Temperature used during treatment / exposure: Room temperature
- Temperature of post-treatment incubation (if applicable): 37 °C
REMOVAL OF TEST MATERIAL AND CONTROLS
-Volume and number of washing steps: Not reported
- Observable damage in the tissue due to washing: None reported
- Modifications to validated SOP: None
MTT DYE USED TO MEASURE TISSUE VIABILITY AFTER TREATMENT / EXPOSURE
- MTT concentration: 2 mL MTT-solution (0.3 mg/mL in PBS)
- Incubation time: 3 hours
- Spectrophotometer: TECAN Infinite® M200 Pro Plate Reader
- Wavelength: 570 nm
- Filter: None reported
- Filter bandwidth: Not applicable
- Linear OD range of spectrophotometer: Not reported
NUMBER OF REPLICATE TISSUES: 3
CONTROL TISSUES USED IN CASE OF MTT DIRECT INTERFERENCE : The test item was checked for possible color interference and direct MTT reduction before the study was started. Some non-colored test items may change into colored items in aqueous conditions and thus stain the skin tissues during the exposure. To assess the color interference, at least 10 mg of the test item was added to 90 µL Milli-Q water. The mixture was mixed for approximately 15 minutes. A negative control, 10 µL Milli-Q water was tested concurrently. At the end of the shaking period a color check was performed. To assess the ability of the test item to reduce MTT, at least 10 mg of the test item was added to 2 mL MTT solution (0.3 mg/mL in PBS). The mixture was incubated for 3 hours at 37°C. A negative control, 25 µL sterile Milli-Q water was tested concurrently. At the end of the incubation period a color check was performed. Because no color changes were observed it was concluded that A027 did not interact with the MTT endpoint.
NUMBER OF INDEPENDENT TEST SEQUENCES / EXPERIMENTS TO DERIVE FINAL PREDICTION: 1
PREDICTION MODEL / DECISION CRITERIA (choose relevant statement)
- The test substance is considered to be irritant to skin if the relative mean tissue viability of three individual tissues after 15 minutes of exposure to the test item and 42 hours of post incubation is ≤ 50% of the mean viability of the negative controls. - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): The solid test item was applied directly on top of the skin tissue and was spread to match the size of the tissue.
NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 25 µL
- Concentration (if solution): PBS
POSITIVE CONTROL
- Amount(s) applied (volume or weight): 25 µL
- Concentration (if solution): 5% (aq) Sodium dodecyl sulfate in PBS - Duration of treatment / exposure:
- 15 ± 0.5 minutes; the positive control was re-spread after 7 minutes contact time.
- Duration of post-treatment incubation (if applicable):
- 42 hours
- Number of replicates:
- 3
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- Mean
- Value:
- 112
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Other effects / acceptance of results:
- - OTHER EFFECTS:
None reported
- Visible damage on test system: None reported
- Direct-MTT reduction: No color changes were observed, therefore it was concluded that the test item did not interact with the MTT endpoint
- Colour interference with MTT: No color changes were observed, therefore it was concluded that the test item did not interact with the MTT endpoint
DEMONSTRATION OF TECHNICAL PROFICIENCY: All validity criteria met
ACCEPTANCE OF RESULTS:
- Acceptance criteria met for negative control: Yes
- Acceptance criteria met for positive control: Yes
- Acceptance criteria met for variability between replicate measurements: Yes
- Range of historical values if different from the ones specified in the test guideline: See table 4
Any other information on results incl. tables
Table 1: Mean Absorption in the In Vitro Skin Irritation Test
A (OD570) | B (OD570) | C (OD570) | Mean (OD570) | SD | |
Negative Control | 0.998 | 0.972 | 1.039 | 1.003 | 0.034 |
Test item | 1.282 | 1.107 | 0.986 | 1.125 | 0.148 |
Positive Control | 0.042 | 0.055 | 0.041 | 0.046 | 0.008 |
OD = Optical density
SD = Standard Deviation
Values are corrected for background absoprtion (0.042); Isopropanol was used to measure the background absorption
Table 2: Mean Tissue Viability in the In Vitro Skin Irritation Test
Mean tissue viability (% of control) | Standard Deviation (%) | |
Negative Control | 100 | 3.4 |
Test item | 112 | 15 |
Positive Control | 4.6 | 0.8 |
Table 3: Individual OD Measurements at 570 nm
OD570 Measurement | A (OD570) | B (OD570) | C (OD570) | |
Negative Control | 1 | 1.0376 | 1.0264 | 1.0975 |
2 | 1.3237 | 1.1762 | 1.0396 | |
Test item |
1 |
1.3237 |
1.1762 |
1.0396 |
2 |
1.3233 |
1.1207 |
1.0171 |
|
Positive Control |
1 |
0.0809 |
0.0971 |
0.0833 |
2 |
0.0868 |
0.0972 |
0.0824 |
OD = Optical density
Table 4: Historical Control Data for In Vitro Skin Irritation Studies
Negative Control (absorption; OD570) |
Positive Control (absorption; OD570) |
|
Range |
0.422 - 1.547 |
0.023 - 0.437 |
Mean |
0.98 |
0.13 |
SD |
0.18 |
0.08 |
n |
174 |
173 |
SD = Standard deviation
n = Number of observation
Historical control data range of the controls were obtained by collecting all data over the period November 2014 to November 2017
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The relative mean tissue viability obtained after 15 ± 0.5 minutes treatment with the test item compared to the negative control tissues was 112%. Since the mean relative tissue viability for the test item was above 50%, it is considered to be non-irritant.
- Executive summary:
The skin irritancy potential of the test item was conducted in an in vitro study EPISKIN Reconstructed Human Epidermis Model test according to OECD 439 and EU B.46 test guidelines alongside Phosphate Buffer solution (PBS) and 5 % w/v Sodium dodecyl sulphate (SDS) as a negative and positive controls, respectively. The relative mean viability of the test item treated tissues was 112 % after a 15 minute +/- 0.5 minute exposure period followed by 42 hour post-exposure period. The test item was classified as non-irritant under the UN GHS classification criteria.
The study is a GLP compliant guideline experimental study with no restrictions and therefore fully adequate for assessment for this endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.