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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Renewable hydrocarbons, C15-C18, branched alkanes was biodegraded 80% at day 28 in the Closed Bottle test. Hence this substance should be classified as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

In order to assess the biotic degradation in seawater, a biodegradability test was performed which allows the biodegradability to be measured in seawater. The biodegradability was determined in the Closed Bottle test performed according to slightly modified OECD Test Guidelines, and in compliance with the OECD principles of Good Laboratory Practice.

Renewable hydrocarbons, C15-C18, branched alkanes did not cause a reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum.

Renewable hydrocarbons, C15-C18, branched alkanes was biodegraded 83% at day 28 in the Closed Bottle test. Hence this substance should be classified as readily biodegradable.

The test is valid as shown by an endogenous respiration of 1.0 mg/L and by the total mineralisation of the reference compound, sodium acetate. Sodium acetate was degraded 81% of its theoretical oxygen demand after 28 days. Finally, the most important criterion was met by oxygen concentrations >0.5 mg/L in all bottles during the test period.