Reaction mass of Terpenes and terpenoids, turpentine-oil, beta-pinene fraction, dimers and Terpenes and terpenoids, turpentine-oil, beta-pinene fraction, trimers

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 March - 16 August 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

other: Slow-stir methodology

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material: AN-0400-112
- Expiration date of the lot/batch: 01 January 2019
- Purity: 100%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Frozen at approximately -20 °C, under nitrogen, in the dark

Key result

Water solubility:

0 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

0.25 g/L

Incubation duration:

1 225 h

Temp.:

20 °C

pH:

6.4 - 7

Details on results:

The preliminary estimate was 3.40 x10^-20 g/L at 25°C.
Vessel 1 - mean concentration: 9.91 x10^-6 g/L
Vessel 2 - mean concentration: 1.01 x 10^-5 g/L
Vessel 3 - mean concentration: 9.48 x 10^-6 g/L
A mean of these three results were used to determine the overall water solubility of the test substance, giving a value of 9.84 x10^-6 g/L

The linearity of the detector response with respect to concentration was assessed over the nominal concentration range of 0.141 - 1.41 mg/L on the GC-MS (equivalnet to 2.8 x 10^-6 - 2.8 x10^-5 g/L in the aqueous sample prior to extraction for analysis). The results were satisfactory with a first order correlation coefficient (r) of 0.999 being obtained. The graph can be found in the 'Attached background material' section.

Conclusions:

The water solubility of the test substance, Terpenes and terpenoids, turpentine oil, beta-pinenefraction polymerised, was determined to be 9.84 x 10^-6 g/L.

Executive summary:

The water solubility of the test substance, Terpenes and terpenoids, turpentine oil, beta-pinene fraction polymerised was determined via a slow-stir methodology via a procedure compatible with Method A6 Water Solubility of Commission Regulation (EC) NO. 440/2008 of 30 May 2008, Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995 and Method 830.7840 of the OCSPP Guidelines, March 1998.

A preliminary test was initially conducted in order to provide an estimation of the water solubility of the test substance via WSKOW software. In the definitive test, 4 replicate jacketed glass vessels (preliminary flask to establish equilibrium period and three for the main test) were filled with 4000 mL purified water. Aliquots of the test substance were added to the surface of each vessel and then stirred at a speed as not to cause a physical vortex visible on the surface of the solutions.  The test vessels were protected from light during stirring. For sampling, stirring was stopped and approximately 15 mL solution was dispensed to waste and the pH of each sampling timepoint was recorded. For Priminart flask samples 1 and 2,  duplicate aliquots (200 mL) from each vessel were sampled for analysis.  For all other samples, a single 200 mL aliquot was sampled. Following sampling for analysis, stirring was re-started. The preliminary flask alone was sampled and analyzed initially in order to establish the time required to achieve the saturation equilibrium. All three main test vessels were analyzed until a saturation plateau was confirmed analytically for each main test individual vessel.

The results from the triplicate vessels were used to determine the water solubility for the test substance, that was calculated to be 9.84 x 10^-6 g/L at a temperature of 20.0 °C ± 0.5°C.

Description of key information

The water solubility of the test substance, Terpenes and terpenoids, turpentine oil, beta-pinene fraction polymerised was determined via a slow-stir methodology via a procedure compatible with Method A6 Water Solubility of Commission Regulation (EC) NO. 440/2008 of 30 May 2008, Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995 and Method 830.7840 of the OCSPP Guidelines, March 1998.

A preliminary test was initially conducted in order to provide an estimation of the water solubility of the test substance via WSKOW software. In the definitive test, 4 replicate jacketed glass vessels (preliminary flask to establish equilibrium period and three for the main test) were filled with 4000 mL purified water. Aliquots of the test substance were added to the surface of each vessel and then stirred at a speed as not to cause a physical vortex visible on the surface of the solutions.  The test vessels were protected from light during stirring. For sampling, stirring was stopped and approximately 15 mL solution was dispensed to waste and the pH of each sampling timepoint was recorded. For Priminart flask samples 1 and 2,  duplicate aliquots (200 mL) from each vessel were sampled for analysis.  For all other samples, a single 200 mL aliquot was sampled. Following sampling for analysis, stirring was re-started. The preliminary flask alone was sampled and analyzed initially in order to establish the time required to achieve the saturation equilibrium. All three main test vessels were analyzed until a saturation plateau was confirmed analytically for each main test individual vessel.

The results from the triplicate vessels were used to determine the water solubility for the test substance, that was calculated to be 9.84 x 10^-6 g/L at a temperature of 20.0 °C ± 0.5°C.

Key value for chemical safety assessment

Water solubility:

0.01 mg/L

at the temperature of:

20 °C