Fatty acids, C18-36, esters with ethylene glycol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 January 2018 to 18 January 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

no analytics were performed because it was not possible to develop a suitable analytical method

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Remarks:

it was not possible to develop a suitable analytical method for this complex UVCB

Vehicle:

no

Details on test solutions:

The 100 mg/L WAF was prepared by adding a nominal 0.2 g of test substance (actual weight: 0.20008 g) to 2000 mL of test media. The control was treated the same without the addition of test substance.
The solutions were stirred for 24 hours and left to settle for approximately 1 hour 15 mins. Undissolved test substance was observed on the surface of the solution, on the sides and bottom of the vessel, in the outlet tube and a large amount was observed in the protruding vessel tap area. Fine particles of test substance were visible throughout the solution. The first ~100 mL drawn off the vessel went to waste to clear the outlet, followed by the water-soluble portion from the middle of the vessel. The solutions were higher than the test temperature due to stirring, so were cooled using an ice bath to a nominal temperature of 20°C.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphmia magna
- Source: laboratory cultures held at Scymaris.
- Age of parental stock (mean and range, SD): 22 ± 1 days (daphnids in test < 24 h)
- Feeding during test: none

ACCLIMATION
- Stock conditions (same as test or not): yes
- Type and amount of food: mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcapitata, strain CCAP 278/4
- Feeding frequency: daily ad libitum
- Health during acclimation: no evidence of diease before test:

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

248.7 as CaCO2

Test temperature:

18.7-20.6 ˚C

pH:

7.64 - 7.94

Dissolved oxygen:

8.96-9.08 mg/L

Nominal and measured concentrations:

nominal: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers of 250 mL
- Type (delete if not applicable):closed
- Fill volume: 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS: The reconstituted water medium used for testing (and maintenance of stock cultures) was Elendt's M4 D. magna medium

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8

EFFECT PARAMETERS MEASURED : immobilisation at 24 and 48 h

VEHICLE CONTROL PERFORMED: NA

RANGE-FINDING STUDY: not performed, 100 mg/L WAFwas prepared

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Based on immobility compared to the control (from direct observation of the data), the 48 hour No Observed Effect Concentration (NOEC) was determined to be 100 mg/L and the Lowest Observed Effect Concentration (LOEC) was >100 mg/L.
There was no immobility observed in the dilution water control. No other symptoms of toxicity were observed.

Reported statistics and error estimates:

Linear interpolation (ICPIN)

Daphnia magna response

Time (hour)	Nominal concentration of the substance (mg/L)	Number immobilised per replicate				Total number tested	Total number immobilised	Percentage immobilised
		A	B	C	D
24	Control	0	0	0	0	20	0	0
	100	0	0	0	0	20	0	0
48	Control	0	0	0	0	20	0	0
	100	0	0	0	0	20	0	0

 

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for Daphnia magna is > 100 mg/L (nominal)

Executive summary:

Daphnids were exposed during 48 h (static test) to control and 100 mg/L (WAF).

Based on immobility compared to the control (from direct observation of the data), the 48 hour No Observed Effect Concentration (NOEC) was determined to be 100 mg/L. The EC50 is > 100 mg/L

There was no immobility observed in the dilution water control. No other symptoms of toxicity were observed.

Description of key information

Daphnids were exposed during 48 h (static test) to control and 100 mg/L (WAF)

Based on immobility compared to the control (from direct observation of the data), the 48 hour NOELR was determined to be 100 mg/L. The EL50 is > 100 mg/L.

There was no immobility observed in the dilution water control. No other symptoms of toxicity were observed.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L