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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
2004-11-23 to 2004-12-03
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2011

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
yes
Remarks:
The water accommodated fraction (WAF) was not prepared for each treatment concentration by weighing the respective load, but by preparation of a WAF of the highest tested concentration (100 mg/L). By using this solution the treatments were prepared.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Dicopper hydroxide phosphate
EC Number:
235-285-2
EC Name:
Dicopper hydroxide phosphate
Cas Number:
12158-74-6
Molecular formula:
Cu2HO5P
IUPAC Name:
copper(2+) hydroxide phosphate (2:1:1)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was stored in a closed vessel at room temperature. Due to the poor water solubility, the water-accommodated fractions of the concentrations to be tested were prepared. This was done by shaking the nominal load with the appropriate amount of dilution water for 24 hours, followed by filtration. The resulting solution was used for treatments to be tested.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation: 0-24 h
- Food type: green alga (Desmodesmus subspicatus)
- Conditions: Kept in M4-Medium following ELENDT, five adults per liter and transferred into fresh medium twice a week

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Observation at 24 and 48 hours

Test conditions

Test temperature:
20 ± 2 °C
pH:
7.9 - 8.2
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
Main study (nominal): 100, 46, 22, 10, 4.6, 2.2 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50-mL-beakers (DIN, HF)
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic dilution water following ISO 6341

OTHER TEST CONDITIONS
- Photoperiod: lighting cycle 16h/8h (light/dark)

EFFECT PARAMETERS MEASURED: 24h and 48h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.17

- Range finding study
- Test concentrations (mg/L): 100, 10, 1, 0.1, 0.01, 0.001

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
22.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
None
Results with reference substance (positive control):
Validity
The 24h-EC50i of the reference K2Cr2O7 should lie between 0.6 and 2.4 mg/L. A 24h-EC50i of 1.2 mg/L was determined in a study performed recently.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 24 and 48 hour NOEC of dicopper hydroxide phosphate was determined to be 4.6 mg/L (nominal).

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