Extract obtained from defatted powder of Theobroma cacao (Malvaceae) by extraction with ethanol

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18-21 September 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 15 and 16 November 2017 / signed on 15 May 2018

Type of method:

pycnometer method

Remarks:

with a replacement liquid

Key result

Type:

relative density

Density:

1.24 other: unitless

Temp.:

20 °C

Key result

Type:

density

Density:

1.24 g/cm³

Temp.:

20 °C

Table 4.4/1: Measured Values

 

First Determination				Error rel.	Error abs.
Temperature	20.1	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.2278	61.7828	56.3605	42.7062	58.7234
Temperature in °C	20.2	20.1	20.2	20.2	20.0
Volume of Pycnometer	25.6010	cm^3
Density of Replacement Liquid	0.7864	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.2379	g/cm^3		0.0437%	0.0005
Second Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.2276	61.7790	56.3585	43.0830	58.769
Temperature in °C	20.0	20.0	20.1	20.2	20.2
Volume of Pycnometer	25.5974	cm^3
Density of Replacement Liquid	0.7864	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.2431	g/cm^3		0.0417%	0.0005

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.2379	0.0005
2	1.2431	0.0005

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	25.60	cm^3	0.0026	0.01%
Density of Test Item	1.2405	g/ cm^3	0.0037	0.30%
Density of Test Item	1240.5	Kg/m^3	3.7	0.30%
Relative Density of Test Item (D20/4)	1.2405		0.0037	0.30%

The relative density of the test item has the same number value, but no unit, assuming that relative density is given as the ratio of test item density to density of water at 4°C, having a standard density of 0.999975 g/cm3 (source: CRC Handbook of Chemistry and Physics - 90th edition / Section 6 - Fluid properties - p.6 -7).

The density of the test item was calculated as 1.2405 ± 0.0005 g/cm³ at 20.0 ± 0.2 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was calculated as 1.2405 ± 0.0005 g/cm3 at 20.0 ± 0.2 °C and the relative density was determined as D20/4 = 1.2405
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method (with a replacement liquid).

Two determinations were performed and the mean value was retained.

This result was then converted to relative density.

Mean density: 1.2405 g/cm³at 20.0 ± 0.2 °C

Mean relative density (D20/4) : 1.2405

Description of key information

The density of the test item was measured as 1.2405 ± 0.0005 g/cm3 at 20.0 ± 0.2 °C.

D20/4 = 1.2405

Key value for chemical safety assessment

Relative density at 20C:

1.24

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method with a replacement liquid, is available. It is considered as a key study, and the result is retained as key data.