2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-03-17 to 2016-04-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

OECD Guideline No. 105 for the testing of chemicals, 27 July 1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

Regulation (EC) No 440/2008, method A.6

Deviations:

no

GLP compliance:

yes

Type of method:

flask method

Key result

Water solubility:

ca. 579.9 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

1 650 g/L

Incubation duration:

<= 3 d

Temp.:

20 °C

pH:

ca. 10

Water solubility:

ca. 516.6 g/L

Conc. based on:

test mat.

Loading of aqueous phase:

1 320 g/L

Incubation duration:

<= 3 d

Temp.:

10 °C

pH:

ca. 9.9

Details on results:

The preliminary test showed a water solubility of the test item of about 1000 g/L at 20 °C.
The main test showed a mean water solubility of the test item of 516.6 g/L at 10 °C, with a standard deviation of 3.75, and 579.9 g/L at 20 °C, with a standard deviation of 1.24.

Water solubility at 10 °C, pH 9.9

Equilibration time (Days)	Measurement 1	Measurement 2	Measurement 3	Mean
1	519.4	506.1	524.2	516.6
2	527.9	511.8	521.3	520.3
3	520.9	504.9	512.7	512.8
Mean				516.6
Standard deviation				3.75

 

Water solubility at 20 °C, pH 10.0

Equilibration time (Days)	Measurement 1	Measurement 2	Measurement 3	Mean
1	584.6	578.6	580.7	581.3
2	582.5	572.0	582.4	579.0
3	586.2	573.1	579.2	579.5
Mean				579.9
Standard deviation				1.24

Conclusions:

The water solubility of the test item was determined to be 579.9 g/L at 20 °C and pH 10.

Executive summary:

The water solubility was determined according to Regulation (EC) No 440/2008, method A.6 and OECD Guideline No. 105, adopted on 27th July 1995 using the shake flask method with HPLC analysis. A preliminary test was undertaken to determine the approximate water solubility. Based on the preliminary result, aliquots of the test item, were weighed into six separate flasks. The water solubility was determined at two different temperatures, 10 °C and 20 °C. The contents of the flasks were filtered and the concentrations were determined by HPLC after standing at 20 °C for a period of not less than 24 hours. The water solubility of the test substance was determined to be 516.6 g/L at 10 °C (pH = 9.9) and 579.9 g/L at 20 °C (pH = 10).

Description of key information

The water solubility of the test item was determined to be 579.9 g/L at 20 °C and pH 10 (reference 4.8-1).

Key value for chemical safety assessment

Water solubility:

579.9 g/L

at the temperature of:

20 °C

Additional information

The water solubility was determined according to Regulation (EC) No 440/2008, method A.6 and OECD Guideline No. 105, adopted on 27th July 1995 using the shake flask method with HPLC analysis. A preliminary test was undertaken to determine the approximate water solubility. Based on the preliminary result, aliquots of the test item, were weighed into six separate flasks. The water solubility was determined at two different temperatures, 10 °C and 20 °C. The contents of the flasks were filtered and the concentrations were determined by HPLC after standing at 20 °C for a period of not less than 24 hours. The water solubility of the test substance was determined to be 516.6 g/L at 10 °C (pH = 9.9) and 579.9 g/L at 20 °C (pH = 10).