Diethyl sulphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Comparison ot ethanol toxicity to Daphnia magna and Ceriodaphnia dubia tested at two different temperatures: Static acute toxicity test results.

Author:

Takahashi, I.T.,Cowgill, U.M., Murphy, P.G.

Year:

1987

Bibliographic source:

Bull. Environ. Contam. Toxicol. 39:229-236.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ASTM E729-80

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol

EC Number:

200-578-6

EC Name:

Ethanol

Cas Number:

64-17-5

Molecular formula:

C2H6O

IUPAC Name:

ethanol

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

Analytical monitoring was not carried out as the authors estimated losses to be less than 10% over the course of the study.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

TEST ORGANISM
Organisms were mass cultured an acclimated to temperature for at least 10 weeks and maintained in filtered, autoclaved Lake Huron water.
- Source: Source not specified
- Age at study initiation: Neonates.
- Common name: water flea
- Strain: Ceriodaphnia dubai
- Method of breeding: Neonates hatched by isolated gravid females gathered by sieving.
- Habitat change: alternate days
- Feeding during test
- Food type: bacteria free algae (A convolutus). Details on feeding reported in Cowgill (Env Toxicol Chem, 4, 415, 1985 and J Crust Biol 5, 420, 1985)
- Amount: 30000 (+/-2000) cells/ml
- feeding rate 3 mls algae/0.2litre vessel
- Frequency: alternate days

ACCLIMATION
- Acclimation period: 10 weeks
- Acclimation conditions (same as test or not): yes

OTHER
- dissolved oxygen: 8.3 +/- 0.2 mg/l
- pH: Initial: 8.2 (+/-0.1) Final 8.8 (+/-0.6)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

90.3 mg/l as CaCO3.(+/- 4.4)

Test temperature:

24 to 24.9 degree C.

pH:

8.2 (+/-0.1) to 8.4 (+/-0.1)

Dissolved oxygen:

8.4 (+/-0.4) to 10.3 (+/-0.1)

Salinity:

not applicable

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

5 012 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: CI = 4233 - 5913

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In a reliable 48 hour acute toxicity study carried out to an ASTM protocol, Ceriodaphnia dubai were exposed to ethanol at sufficient concentrations to enable an LC50 of 5012mg/l to be calculated based on an end point of mortality and as a geometric mean of 3 separate replicated assays. Based on the results of this study, ethanol would be not be classified toxic to the environment according to the classification system of the EU.

This toxicity study is classified as acceptable and satisfies the requirements as the key study for an invertebrate toxicity study.

The LC50 at 48 h was 5012mg/l