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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Toxic effects of organic solvents on the growth of Chlorella vulgaris and Selenastrum capricornutum.
Author:
El Jay, A.
Year:
1996
Bibliographic source:
Bull. Environ. Contam. Toxicol. 57:191-198.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethanol
EC Number:
200-578-6
EC Name:
Ethanol
Cas Number:
64-17-5
Molecular formula:
C2H6O
IUPAC Name:
ethanol
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: not described

Test organisms

Test organisms (species):
Chlorella vulgaris
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: Chlorella vulgaris
- Source (laboratory, culture collection): Isolated from Lake Geneva in 1980
- Age of inoculum (at test initiation): no data
- Method of cultivation: Axenic using Algal assay Procedure (1971) medium with 15 mg/l NaHCO3 and 12 mg/l K2HPO4 in 500 ml flasks containing 250 ml algal suspension.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
5 d

Test conditions

Test temperature:
21 +/- 1 deg C
pH:
Water chemistry (pH) on test: Not described.
Nominal and measured concentrations:
Test design: Ethanol was tested three times at each concentration (0, 0.05, 0.1, 0.3, 0.5 and 1% v/v).
Method of calculating mean measured concentrations: Only nominal concentrations were used.

Results and discussion

Effect concentrations
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
275 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 of the test substance on the growth rate of Chlorella vulgaris at 72 hours was 275 mg/L.