1-Octanamine, N,N-dimethyl-, N-oxide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 09th, 2005 - July 26th, 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Analytical purity: Not available
- Purity test date: Not available
- Lot/batch No.: 75412
- Expiration date of the lot/batch: Not available
- Appearance: Not reported
- Storage: Not reported

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Secondary sewage effluent was collected from Runcorn East Sewage Works (3rd June 2005). After collection, the inoculum was coarse filtered and allowed to settle for one hour before the supernatant liquid was decanted off for use as the test inoculum.

Duration of test (contact time):

28 d

Initial conc.:

5 mg/L

Based on:

test mat.

Initial conc.:

6.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Nutrient enriched freshwater
- Test temperature: 17.5-18.5°C
- pH adjusted: No
- Continuous darkness: Yes

TEST SYSTEM
- Culturing apparatus: Completely filled, sealed glass BOD bottles of nominal 272 ml volume
- Number of culture flasks/concentration: 2 bottles per sampling day for the test material and the reference material vessels, 3 bottles per sampling day for the control (blank) vessels
- Test performed in closed vessels due to significant volatility of test substance: No
- Test performed in open system: No (closed bottle method)

SAMPLING
- Sampling frequency: Dissolved oxygen measured on sample days 0, 3, 7, 14, 21 and 28
- Sample storage before analysis: Used on day of preparation

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (3 bottles per sampling day)
- Abiotic sterile control: No
- Toxicity control: No

Reference substance:

benzoic acid, sodium salt

Remarks:

2.5mg/l

Parameter:

% degradation (O2 consumption)

Value:

ca. 86.8

Sampling time:

7 d

Remarks on result:

other: 5.0 mg test material/L

Key result

Parameter:

% degradation (O2 consumption)

Value:

> 100

Sampling time:

28 d

Remarks on result:

other: 5.0 mg test material/L

Parameter:

% degradation (O2 consumption)

Value:

ca. 85.1

Sampling time:

7 d

Remarks on result:

other: 6.5 mg test material/L

Key result

Parameter:

% degradation (O2 consumption)

Value:

> 100

Sampling time:

28 d

Remarks on result:

other: 6.5 mg test material/L

Results with reference substance:

A degradation figure of 67.8% after 28 days was obtained for the reference material. This demonstrates that the inoculum was biologically active.

Summary of results

	% Degradability
Material (mgl^-1)	Day 3	Day 7	Day 14	Day 21	Day 28
Mackam C-8 (5.0)	4.8	86.8	92.4	90.4	>100.0
Mackam C-8 (6.5)	3.1	85.1	96.4	92.2	>100.0
Sodium benzoate (2.5)	56.3	62.3	67.3	71.9	67.8

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The biodegradation of the test material within 28 days was >100% COD (mean of two replicates) at both 5.0 and 6.5 mg/L, and reached the criteria for ready biodegradability.

Executive summary:

The aerobic biodegradability of the test material was investigated in a GLP-compliant ready biodegradability study performed in accordance with OECD Guideline No. 301D. The biodegradation of the test material within 28 days was >100% COD (mean of two replicates) at both 5.0 and 6.5 mg/L, and reached the criteria for ready biodegradability.

Description of key information

The aerobic biodegradability of the test material was investigated in a GLP-compliant ready biodegradability study performed in accordance with OECD Guideline No. 301D. The biodegradation of the test material within 28 days was >100% COD (mean of two replicates) at both 5.0 and 6.5 mg/L, and reached the criteria for ready biodegradability.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

The aerobic biodegradability of the test material was investigated in a GLP-compliant ready biodegradability study performed in accordance with standard methods, without deviations. The study is considered as reliable (Klimisch 1) and is selected as a key study for the endpoint.