D-glucopyranose, oligomers, xylityl glycosides and 1,4 anhydro D-xylitol and D-xylitol

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2007-02-13 to 2007-02-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study, OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

AND Directive CE - methode C19 et OCDE 121

Deviations:

yes

Remarks:

The deviation was not considered to have compromised the validity or integrity of the study.

GLP compliance:

yes (incl. QA statement)

Remarks:

2005-12-16

Type of method:

HPLC estimation method

Media:

soil

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

446-990-1

EC Name:

-

Molecular formula:

C5H11O5 [C6H10O5]1.45 and C5H10O4 and C5H12O5

IUPAC Name:

2-(hydroxymethyl)oxolane-3,4-diol; 5-{[2,3,4,5-tetrahydroxy-6-(xenoniooxy)hexyl]oxy}pentane-1,2,3,4-tetrol; pentane-1,2,3,4,5-pentol

Details on test material:

batch number: 173 JG-1 (at receipt), 173 JG1 (on the analytical certificate)
description: at receipt, gel
on anlaytical cetrificate yellox hard wax
received: 2006-12-12
storage conditions: at room temperature
purity: 99.7%
hydrosolubility 674 g/L at 20°C
expiry date: 2008-01-03

Radiolabelling:

no

Study design

Test temperature:

22 +/- 1°C

HPLC method

Details on study design: HPLC method:

chromatographic conditions
pump: 118 (Beckman)
Mobile phase (degazed): methanol 55%, water 45%
pH of mobile phase: 5.5
Flow rate: 1 mL/min
Column: Lichrospher 5 CN (Interchim)
particle size: 5um, length= 250mm
inner diameter: 4mm
Detector: Refractive Index Detector 2414 (Waters)
Injector: Spectra system AS3000 (TSP)
Injected volume: 50 uL
Software: Empower 2 (Waters)
Analysis time: 5 min

Batch equilibrium or other method

Computational methods:

As the retention time of the peak observed for the test item was close to the dead volume and below the retention time of acetanilide, reference item (Koc=1.25), the adsorption coefficient was demonstrated to be such as log Koc<1.25. In addition, when possible, an estimation using a calculation method on the non validated software PCKOC (v1.66) was performed.
The test item is a mixture.
The absortption coefficient of the test item was estimeted on the basis of the highest obtained value.

Results and discussion

Adsorption coefficient

Type:

Koc

Value:

<= 1

Temp.:

22 °C

Applicant's summary and conclusion

Conclusions:

Koc <= 1 according to an OECD test n°121
No further adsorption/desorption test is needed

Executive summary:

The aim of this study was to assess the absorption/desorption coefficient (Koc) of aquaxyl on soil. The estimation was carried out according to the test guidelines described in OECD guideline n° 121and directive 2001/59/EEC, C.19.

HPLC method was performed on an analytical column packed with a cyanopropyl solid phase containing lipophilic and polar moities. As the test item is not ionizable, a test was performed with the following mobile phase: methanol/water (55/45 v/v) at pH5.5

As the retention time of the peak observed for the test item was close to the dead volume and below the retention time of acetanilide, reference item (Koc=1.25), the adsorption coefficient was demonstrated to be such as log Koc<1.25. In addition, when possible, an estimation using a calculation method on the non validated software PCKOC (v1.66) was performed.

The test item is a mixture.

The absortption coefficient of the test item was estimeted on the basis of the highest obtained value.

the Koc of the test item was stated ≤1.