3,3'-[azoxybis[(2-methoxy-p-phenylene)azo]]bis[4,5-dihydroxynaphthalene-2,7-disulphonic] acid, lithium sodium salt

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Strain: Daphnia magna, Strauss (Cladocera, Crustacea)
Age: ≤ 24-hours life, in good physiological conditions
Origin: Stirling University, United Kingdom

Culture conditions: The cultivation of daphnia from which the Individuals to carry out the test has been done with the following conditions:
- Temperature: between 18 and 22 ºC
- Photoperiod of 16 hours of light / 8 hours of darkness
- No aeration
- Feeding: Type of food: Chlorella (freshwater microalgae) and marine algae extract
- Frequency of feeding: 3 times / week

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 ± 2 ºC

pH:

Average pH value of culture at the beginning of the test: 7.66
pH value of culture medium at the end of the test: 7.73
pH Minimum concentration at the start of the test: 7.66
pH Minimum concentration at the end of the test: 7.76
pH value maximum concentration at start of test: 7.03
pH value maximum concentration at the end of the test: 7.48

Dissolved oxygen:

Dissolved O2 concentration values (mg / L):
Dissolved O2 value culture medium at the beginning of the test: 8.52
Dissolved O2 value culture medium at the end of the test: 8.42
Dissolved O2 value minimum concentration at start of test: 8.51
Dissolved O2 value minimum concentration at end of test: 8.42
Dissolved O2 value maximum concentration at start of test: 8.48
Dissolved O2 value maximum concentration at the end of the test: 8.40

Nominal and measured concentrations:

Nominal: 10 mg/L, 22 mg/L, 48 mg/L, 100 mg/L, 220 mg/L, 480 mg/L, 1000 mg/L.

Details on test conditions:

Photoperiod: darkness
Test vessels: Test tubes (40 mL) capped with parafilm.
Test volume used: 10 mL
Number of test vessels per concentration: 4
Number of individuals per concentration: 20, divided into 5 individuals per test vessel
Age of individuals: less than 24 hours of life

Composition of culture medium OECD 202:
- Solution 1: CaCl 2 2H 2 O 11.76 g / L H 2 O
- Solution 2: MgSO 4 7H 2 O 4.93 g / L H 2 O
- Solution 3: NaHCO 3 2.59 g / L H 2 O
- Solution 4: KCl 0.23 g / L H 2 O
Add to 1000 mL of deionized water:
Solution 1 25 mL
Solution 2 25 mL
Solution 3 25 mL
Solution 4 25 mL

Reference substance (positive control):

yes

Remarks:

The reference substance used to verify the response of the test is potassium dichromate (K2Cr2O7). The result of periodic verification of the response of the test organisms is EC 50(24h) = 0.7 mg / L.

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

929 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

929 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Remarks:

The validation criterion considered for this test are: - the percentage of immobilisation observed in the control vessels is less than or equal to 10% - the EC50(24h) of potassium dichromate is between 0.6 mg / L and 1.7 mg / L

Conclusions:

The result obtained in this study indicates that the test substance has an EC50 value of 48h equal to 929 mg / l Daphnia Magna

Executive summary:

In this study of acute toxicity to aquatic invertebrates on the test substance, the Daphnia magna has been used for the determination of inhibition of mobility, according to the guidelines and methods OECD 202 (1984) and Royal Decree 363/1995 (BOE No. 133, June 1995).
The freshwater Daphnia magna has been exposed to the test substance, at the test concentrations: 10 mg/L, 22 mg/L, 48 mg/L, 100 mg/L, 220 mg/L, 480 mg/L, 1000 mg/L act. ingr. The total duration of the test has been 48 hours.
The result obtained in this test indicates that the test substance has an EC50 value (48h) equal to 929 mg / L act.ingr.

Description of key information

There is one study available to determine the short-term toxicity of the substance Direct Blue 281 (CAS 85136 -25 -0) to Daphnia according to OECD Guideline 202. At the maximum concentration tested (EC50(daphnia)>900 mg/L act. ingr.) and considering the existing information, the substance is not harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

929 mg/L