Resinoid of Abies balsamea (Pinaceae) obtained from needles by an organic solvent extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 22 to June 04, 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

adopted 02 October 2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

Regulation (EC) No 440/2008 of 30 May 2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 29 and 30 November 2010 / signed on 11 April 2011

Type of method:

pycnometer method

Key result

Type:

density

Density:

1.057 g/cm³

Temp.:

20 °C

Key result

Type:

relative density

Density:

1.057 other: unitless

Temp.:

20 °C

Table 4.4/1: Measured Values

 

First Determination				Error rel.	Error abs.
Temperature	19.8	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.9448	61.8109	61.8032	41.3295	62.0374
Temperature in °C	19.8	19.8	20.2	19.8	20.0
Volume of Pycnometer	24.9109	cm^3
Density of Replacement Liquid	0.9979	g/cm^3
Density of Test Item	1.0542	g/cm^3		0.0474%	0.0005
Second Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liq.	Pycn. + test item	Pycn. + test item + repl. liq.
Mass in g	36.9447	61.8039	61.7974	42.5257	62.1204
Temperature in °C	20.0	20.0	19.8	20.0	19.8
Volume of Pycnometer	24.9040	cm^3
Density of Replacement Liquid	0.9979	g/cm^3
Density of Test Item	1.0592	g/cm^3		0.0384%	0.0004

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables: 

Table 4.4/2: Results of Single Determinations

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.0542	0.0005
2	1.0592	0.0004

 

Table 4.4/3 Results – Mean Values

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	24.91	cm^3	0.0049	0.02%
Density of Test Item	1.0567	g/ cm^3	0.0036	0.34%
Density of Test Item	1056.7	Kg/m^3	3.6	0.34%
Relative Density of Test Item	1.0567		0.0036	0.34%

 

The density of the test item was calculated as 1.0567 ± 0.0005 g/cm³ at 20.0 ± 0.2 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was calculated as 1.0567 ± 0.0005 g/cm3 at 20.0 ± 0.2 °C and the relative density is determined as D20/4 = 1.0567 ± 0.0005
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density towards water at 4°C.

Mean density: 1.0567± 0.0005 g/cm³at 20.0 ± 0.2 °C

Mean relative density (D20/4) : 1.0567

Description of key information

The density of the test item was calculated as 1.0567 ± 0.0005 g/cm3 at 20.0 ± 0.2 °C  and the relative density is determined as D20/4 = 1.0567

Key value for chemical safety assessment

Relative density at 20C:

1.057

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method, is available. It is considered as a key study, and the result is retained as key data.