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Endpoint summary

Additional information

Short term toxicity to aquatic invertebrates

Immobilization of Daphnia magna exposed to the test item was investigated during a 48-hour static test according to OECD guideline 202. The test was performed in glass beakers of 150 mL capacity, containing 100 mL of each treatment per replicate. The following test item concentrations were used: 1.0, 0.45, 0.20, 0.091, 0.041, 0.019, 0.0086, 0.0039, 0.0018 and 0.00082 mg/L plus the control. The Daphnia magna were observed for immobilization after 24 and 48 h of exposure. The Daphnia magna were considered immobile if they showed no ability to swim within 15 seconds after gentle agitation of the test vessel. In the control and in the test item concentrations of 0.00082, 0.0018, 0.0039, 0.0086 mg/L no immobilization was observed during exposure. At exposure termination in the test item concentrations of 0.019 and 0.041 mg/L immobilization was 15 and 85%, respectively. In the test item concentrations 0.091, 0.20, 0.45 and 1.0 mg/L the immobilization was 100%. The endpoint values determined based on the nominal test item concentrations after 48 h of exposure are given below:

The EC50/48 h value is 0.028 mg/L (95% confidence interval 0.023 – 0.034).

The LOEC/48 h value is 0.041 mg/L.

The NOEC/48 h value is 0.019 mg/L.

Toxicity to aquatic plants

The growth of Lemna gibba exposed to the test item was investigated in a 7 day semi-static test with daily renewals according to OECD guideline 221. The test was performed in glass crystallizers containing 150 mL of each treatment per replicate. Seven test item concentrations of 1.0, 0.2, 0.04, 0.008, 0.0016, 0.00032, 0.000064 mg/L plus the control were used in the test. At exposure termination, in the test item concentrations of 0.000064, 0.00032 and 0.0016 mg/L no distinctive changes from the normal development of plants in the control were observed. In the test item concentration of 0.008 mg/L, separating of roots and chlorosis were observed. In the test item concentration of 0.04 mg/L, separating of roots, bending down of colonies and chlorosis were observed. In the test item concentration of 0.2 mg/L, separating of roots, colored roots, bending down of colonies, breaking up of colonies and chlorosis were observed. In the test item concentration of 1.0 mg/L, separating of roots, colored roots and fronds, bending down of colonies, breaking up of colonies and necrosis were observed.

After 7 days an ErC50 of 0.044 mg/L (95% CI 0.0024 - 0.0082) and ErC10 of 0.0003 mg/L (95% CI 0.0001 - 0.0009) was determined based on frond number.

Toxicity to microorganisms

A 3-hours Activated Sludge Respiration Inhibition Test was conducted according to OECD guideline 209 (BASF 08G0495/14G370, 2015). Activated sludge from a municipal wastewater treatment plant was exposed to the nominal test substance concentrations of 62.5, 125, 250, 500 and 100 mg/L  (without correction of purity of the test substance). 3,5 dichlorophenol was used as a positive control and blank controls were included in the test. After 3 hours of exposure the inhibition of oxygen consumption was determined. The EC50 of 3,5-dichlorophenol was 5.9 mg/L. For the test substance an EC10 of 110 mg/L (95% CL: 64.2-196.2) and an EC50 of 360 mg/L (95%-CL: 277.0 – 470.0) were determined.