Registration Dossier
Registration Dossier
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EC number: 252-563-9 | CAS number: 35441-13-5
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to aquatic invertebrates
EC50 > 100 mg/l nominal concentration
Toxicity to aqutic plants other than algae
ErC50 > 100 mg/l based on nominal concentration (corresponding to 0.016 mg/l measured)
Additional information
Short-term toxicity to aquatic invertebrates
A non-GLP preliminary experiment was carried out at saturation concentration to determine the approximate toxicity of the test item. For preparation of the test solution a supersaturated solution (100 mg/L nominal loading).Based on the results of this non-GLP preliminary test, the test item Fluorescent Brightener 313 had no toxic effect on Daphnia magna at aquatic saturation. The 48h EC50, and NOEC values can be determined to be higher than saturation.
Toxicity to aqutic plants other than algae
The substance was tested on Lemna Gibba according to the OECD guideline 221. A limit test was conducted at a single concentration at saturation (corresponding to 100 mg/L nominal concentration) using concurrent control ran. The exposure concentration of the saturated test solution was analytically determined to be 0.016 mg/L (calculated as the geometric mean of start and end concentrations measured at each renewal period).
The plants were exposed to the test substance for 7 days in a semi-static system (24-h water renewal periods).
In this 168-hour semi-static growth inhibition test onLemna gibbathe test item Fluorescent Brightener 313 had no toxic effect at aquatic saturation (measured value: 0.016 mg/L; equivalent to 100 mg/L nominal concentration).
Accordingly, the 168-h EC10, EC20, EC50 and the LOEC values based on growth rate and yield were determined to be > 0.016 mg/L (equivalent to 100 mg/L nominal concentration). The 168-h NOEC values were determined to be 0.016 mg/L (equivalent to 100 mg/L nominal concentration).
All validity criteria were met.
Based on the infromation availabale the test substance doesn't show any concern for aquatic environment therefore, it was not classified according to the CLP Regulation (EC) No 1272/2008.
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