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Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 > 100 mg/l nominal concentration

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

According to the REACH Regulation n. 1097/2006, Annex VII, Column 2: " The study does not need to be conducted if there are mitigating factors indicating that aquatic toxicity is unlikely to occur, for instance if the substance is highly insoluble in water or the substance is unlikely to cross biological membranes".

The substance is highly insoluble in water, therefore this study should have been waived. However, a non-GLP preliminary experiment was carried out at saturation concentration to determine the approximate toxicity of the test item. For preparation of the test solution a supersaturated solution (100 mg/L nominal loading).Based on the results of this non-GLP preliminary test, the test item Fluorescent Brightener 313 had no toxic effect on Daphnia magna at aquatic saturation. The 48h EC50, and NOEC values can be determined to be higher than saturation.