N-ethyl-p-methoxy-α-methylphenethylamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: refer principle below

Principles of method if other than guideline:

WoE derived based on the predicted data for the target chemical and experimental data from structurally and functionally similar read across chemicals

1. Biodegradation study was conducted for 20 days for evaluating the percentage biodegradability of test chemical.

2. Biodegradability of test chemical was determined by considering degradative oxidation as parameter and Beauveria bassiana (ATCC 7 159) as inoculums for 3 days.

3.Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301C “Ready biodegradability: Modified MITI Test (I)”.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: N-ethyl-p-methoxy-α-methylphenethylamine
- IUPAC name: N-ethyl-1-(4-methoxyphenyl)propan-2-amine
- Molecular formula: C12H19NO
- Molecular weight: 193.2881 g/mole
- Smiles : CCNC(C)Cc1ccc(OC)cc1
- Inchl: 1S/C12H19NO/c1-4-13-10(2)9-11-5-7-12(14-3)8-6-11/h5-8,10,13H,4,9H2,1-3H3
- Substance type: Organic
- Physical state: Liquid (Colorless to pale yellow)

Oxygen conditions:

aerobic

Inoculum or test system:

other: 1. Bacteria 2. Beauveria bassiana fungus (ATCC 7159) 3. Activated sludge

Details on inoculum:

1. Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Microbial inoculum was isolated from Hudson Collamer silt loam
Concentration of sludge: 5 mg

2. No data

3. Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sludge and surface water including surface soil were collected from ten different places in Japan which includes water treatment plants, rivers, lake and inner bays. These sludge and water/soil were mixed and cultivated with glucose and peptone as nutrient in a testing laboratory.
Concentration of sludge: 30 mg/l as suspended solids

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Initial conc.:

1 000 mg/L

Based on:

test mat.

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

other: Other degradative oxidation

Parameter followed for biodegradation estimation:

other: BOD, HPLC and TOC removal

Details on study design:

1. TEST CONDITIONS
- Test temperature: 25ᵒC

TEST SYSTEM
- Culturing apparatus: BOD bottles
- Measuring equipment: Oxygen analyzer
- Other: The bottles were filled with the air saturated salt solution and closed with the help of a glass stoppers.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Bottles containing O2 saturated water inoculated with soil (as a source of microbial inoculum) but no carbon source were also included in the study to account for the O2 depletion resulting from microbial oxidation of organic matter and ammonium.
- Toxicity control: Test compound was also tested in combination with glucose (both at a conc. of 2 mg of carbon per bottle) to test whether the possible lack of biodegradation was a result of toxicity of the test chemical.


2. TEST CONDITIONS
- Composition of medium:
T 1corn step atomised 12 g/l,
D-glucose 10 g/l,
agar 30 g/l,
pH 5.5.
T3, bacto-triptone 10 g/l,
K2HPO4 1 g/l, D-glucose 30 g/l, FeSO4, .
7H2O 0.01 g/l, MgSO4 7H2O 0.5 g/l, ZnSO47H2O 0.3 g/l, KCl 0.5 g/l, pH 7.2. MPGB, D-glucose 20 g/l, peptone 5 g/l, malt 20 g/l.

- Test temperature: 30 oC

- Inoculation procedure: 5 ml of T1 medium were seeded with the microorganism and incubated for 4 days at 30°C. The biomass was suspended in 4 ml of T3 medium and 2 ml of this suspension was inoculated in 50 ml of the same medium and shaken at 180 rpm for 24 h at 30°C. 5 ml of this culture was inoculated in 50 ml of fresh T3 medium and incubated for 3 days in the same conditions. 3
ml of the content of the flask was transferred in 50 ml of MPGB medium and shaken at 180 rpm at 30°C for 24 h. At this point 50 mg of solid substrates 4-methoxyphenylacetone
was added and the mixture stirred at 180 rpm for 72 h at 30°C.

3. Standard type

TEST CONDITIONS
- Additional substrate: No
- Solubilising agent: Not used

TEST SYSTEM
- Measuring equipment: BOD meter

SAMPLING
- Sampling method: BOD was continuously measured by BOD analysis over 14 days. Direct analysis by HPLC and TOC analysis were conducted after 28 days.

Parameter:

other: degradative oxidation

Value:

28

Sampling time:

3 d

Remarks on result:

other: other details not available

Parameter:

other: % degradation BOD

Value:

98

Sampling time:

28 d

Remarks on result:

other: other details not available

Parameter:

% degradation (TOC removal)

Value:

98

Sampling time:

28 d

Remarks on result:

other: other details not available

Parameter:

% degradation (test mat. analysis)

Remarks:

HPLC

Value:

100

Sampling time:

28 d

Remarks on result:

other: other details not available

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

Biodegradation in water of test chemical N-ethyl-p-methoxy-α-methylphenethylamine( CAS no. 14367-73-5) was done by using experimental data from read across chemicals in all the studies of read across chemical showed that percent biodegradability of test chemical is greater than 70 % . on the basis of percent biodegradability it is concluded that test chemical N-ethyl-p-methoxy-α-methylphenethylamine is readily biodegradable in nature.

Executive summary:

Biodegradation in water of test chemical was predicted for N-ethyl-p-methoxy-α-methylphenethylamine using data from structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

First biodegradation study was conducted for 20 days for evaluating the percentage biodegradability of test substance. Bacteria were used as an inoculum. Microbial inoculum was isolated from Hudson Collamer silt loam. The test was performed under aerobic conditions at a temperature of 25ᵒC, respectively. The chemicals were introduced into the BOD bottles as sole carbon sources at a concentration of 2 mg of carbon per bottle. The compounds were added in acetone solutions, and the acetone was evaporated prior to the addition of O2-saturated water. Each bottle received 5 mg of Hudson Collamer silt loam as a source of the microbial inoculum. The bottles were filled with the air-saturated salts solution and closed with glass stoppers. Bottles containing O2 saturated water inoculated with soil (as a source of microbial inoculum) but no carbon source were also included in the study to account for the O2 depletion resulting from microbial oxidation of organic matter and ammonium. Test compound was also tested in combination with glucose (both at a conc. of 2 mg of carbon per bottle) to test whether the possible lack of biodegradation was a result of toxicity of the test chemical. Dissolved O2 in the bottles was measured at regular intervals using a Yellow Spring Instrument Co. oxygen analyzer, Model 53.The instrument was calibrated with the salts solution, theO2content of which was determined by the Alsterberg modification of the Winkler method. At regular intervals, the dissolved O2 in the samples was measured after calibrating the instrument with a BOD bottle containing inoculated 02-saturated water supplemented with 0.1% KCN. The solutions in bottles showing O2 depletion were used to obtain microorganisms capable of utilizing the substrate. Based on appreciable degradation of test chemical after only a few days, test chemical is considered to be biodegradable in nature.

 

In next study the biodegradation experiment was performed for test chemical by using Beauveria bassiana (ATCC 7 159) fungus as inoculums for 3 days following procedure was used in this experiment.

5 ml of T1 medium were seeded with the microorganism and incubated for 4 days at 30°C. The biomass was suspended in 4 ml of T3 medium and 2 ml of this suspension was inoculated in 50 ml of the same medium and shaken at 180 rpm for 24 h at 30°C. 5 ml of this culture was inoculated in 50 ml of fresh T3 medium and incubated for 3 days in the same conditions. 3

ml of the content of the flask was transferred in 50 ml of MPGB medium and shaken at 180 rpm at 30°C for 24 h. At this point 50 mg of solid substrates4-methoxyphenylacetone

was added and the mixture stirred at 180 rpm for 72 h at 30°C.The test chemical undergoes 28 % degradation by considering degradative oxidation as parameter and Beauveria bassiana (ATCC 7159) as inoculums in 3 days. On the basis of percent degradability value it can be concluded that test chemical is readily biodegradable.

 

In last study the biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301C “Ready biodegradability: Modified MITI Test (I)” under aerobic conditions. Activated sludge was used as a test inoculum. Sludge and surface water including surface soil were collected from ten different places in Japan which includes water treatment plants, rivers, lake and inner bays. These sludge and water/soil were mixed and cultivated with glucose and peptone as nutrient in a testing laboratory. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. Aniline was used as a reference substance for the study. Portion of the test solution was taken out followed by the centrifuge separation. Then, the supernatant solution was employed for the quantitative analysis for HPLC and TOC. BOD was continuously measured by BOD analysis over 14 days. Direct analysis by HPLC and TOC analysis were conducted after 28 days. The percentage degradation of test substance was determined to be 99, 98 and 100% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.

 

By considering results of all the studies mentioned above it can be concluded that test chemical N-ethyl-p-methoxy-α-methylphenethylamine is expected to be readily biodegradable in nature.

Description of key information

Biodegradation in water of test chemical N-ethyl-p-methoxy-α-methylphenethylamine( CAS no. 14367-73-5)  was done by using experimental data from read across chemicals in all the studies of read  across chemical showed that percent biodegradability of test chemical is greater than 70 % . on the basis of percent biodegradability it is concluded that test chemical N-ethyl-p-methoxy-α-methylphenethylamine is readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

Biodegradation in water of test chemical was predicted for N-ethyl-p-methoxy-α-methylphenethylamine using data from structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

First biodegradation study was conducted for 20 days for evaluating the percentage biodegradability of test substance. Bacteria were used as an inoculum. Microbial inoculum was isolated from Hudson Collamer silt loam. The test was performed under aerobic conditions at a temperature of 25ᵒC, respectively. The chemicals were introduced into the BOD bottles as sole carbon sources at a concentration of 2 mg of carbon per bottle. The compounds were added in acetone solutions, and the acetone was evaporated prior to the addition of O2-saturated water. Each bottle received 5 mg of Hudson Collamer silt loam as a source of the microbial inoculum. The bottles were filled with the air-saturated salts solution and closed with glass stoppers. Bottles containing O2 saturated water inoculated with soil (as a source of microbial inoculum) but no carbon source were also included in the study to account for the O2 depletion resulting from microbial oxidation of organic matter and ammonium. Test compound was also tested in combination with glucose (both at a conc. of 2 mg of carbon per bottle) to test whether the possible lack of biodegradation was a result of toxicity of the test chemical. Dissolved O2 in the bottles was measured at regular intervals using a Yellow Spring Instrument Co. oxygen analyzer, Model 53.The instrument was calibrated with the salts solution, theO2content of which was determined by the Alsterberg modification of the Winkler method. At regular intervals, the dissolved O2 in the samples was measured after calibrating the instrument with a BOD bottle containing inoculated 02-saturated water supplemented with 0.1% KCN. The solutions in bottles showing O2 depletion were used to obtain microorganisms capable of utilizing the substrate. Based on appreciable degradation of test chemical after only a few days, test chemical is considered to be biodegradable in nature.

 

In next study the biodegradation experiment was performed for test chemical by using Beauveria bassiana (ATCC 7 159) fungus as inoculums for 3 days following procedure was used in this experiment. 5 ml of T1 medium were seeded with the microorganism and incubated for 4 days at 30°C. The biomass was suspended in 4 ml of T3 medium and 2 ml of this suspension was inoculated in 50 ml of the same medium and shaken at 180 rpm for 24 h at 30°C. 5 ml of this culture was inoculated in 50 ml of fresh T3 medium and incubated for 3 days in the same conditions. 3 ml of the content of the flask was transferred in 50 ml of MPGB medium and shaken at 180 rpm at 30°C for 24 h. At this point 50 mg of solid substrates4-methoxyphenylacetone

was added and the mixture stirred at 180 rpm for 72 h at 30°C.The test chemical undergoes 28 % degradation by considering degradative oxidation as parameter and Beauveria bassiana (ATCC 7159) as inoculums in 3 days. On the basis of percent degradability value it can be concluded that test chemical is readily biodegradable.

 

In last study the biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301C “Ready biodegradability: Modified MITI Test (I)” under aerobic conditions. Activated sludge was used as a test inoculum. Sludge and surface water including surface soil were collected from ten different places in Japan which includes water treatment plants, rivers, lake and inner bays. These sludge and water/soil were mixed and cultivated with glucose and peptone as nutrient in a testing laboratory. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. Aniline was used as a reference substance for the study. Portion of the test solution was taken out followed by the centrifuge separation. Then, the supernatant solution was employed for the quantitative analysis for HPLC and TOC. BOD was continuously measured by BOD analysis over 14 days. Direct analysis by HPLC and TOC analysis were conducted after 28 days. The percentage degradation of test substance was determined to be 99, 98 and 100% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.

 

By considering results of all the studies mentioned above it can be concluded that test chemical N-ethyl-p-methoxy-α-methylphenethylamine is expected to be readily biodegradable in nature.