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Registration Dossier
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EC number: 275-640-9 | CAS number: 71566-55-7
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-02-09 - 1988-04-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (Paris 1984)
- Deviations:
- yes
- Remarks:
- , instead a centrifuged sludge a settled sludge was used
- GLP compliance:
- no
Test material
- Reference substance name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxy-N-3-(isopropoxypropyl)benzenesulphonamidato(2-)]cobaltate(1-)
- EC Number:
- 275-640-9
- EC Name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxy-N-3-(isopropoxypropyl)benzenesulphonamidato(2-)]cobaltate(1-)
- Cas Number:
- 71566-55-7
- Molecular formula:
- C44H50CoN10O10S2.C28H31N2O3
- IUPAC Name:
- 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium bis[3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-4-hydroxy-N-3-(isopropoxypropyl)benzenesulphonamidato(2-)]cobaltate(1-)
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Physical state: red powder (solid)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge from a sewage treatment plant (CH - 4153 Reinach)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- 100, 32, 10, 3.2 and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL BOD flasks with gas inlet
- Source: Activated sludge from a sewage treatment plant
- Material, size, headspace, fill volume: the sudge concentration in the test bottles was 1.6 g/L
TEST CONCENTRATIONS
- Test concentrations: 100, 32, 10, 3.2 and 1 m/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The IC50 of the reference substance 3,5-Dichlorophenol is: 11 mg/L
- Reported statistics and error estimates:
- none
Any other information on results incl. tables
| Test item (mg/L) | oxygen-comsumption (mg/L/h) | Inhibition (%) |
| 1.0 | 37.0 | 27 |
| 3.2 | 45.00 | 11 |
| 10.0 | 48.00 | 5 |
| 32.0 | 43.50 | 14 |
| 100.0 | 45.60 | 10 |
| 3,5 -Dichlorophenol | ||
| 3.2 | 47.10 | 7 |
| 10.0 | 24.90 | 51 |
| 32.0 | 9.96 | 80 |
| Control K1, K2 | 52.60; 48.75 | -4; 4 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the degradation activity of activated sludge is not anticipated when introduced to biological treatment plants in appropriate low concentrations.
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