Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988-07-18 - 1988-08-04
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
(1984)
Deviations:
yes
Remarks:
Highest vehicle concentration: 791.4 mg/L
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Physical state: powder (solid)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- stock solution: 5.0 g test item and 20.0 mg alkylphenol-polyglykol-ether were mixed with and made up to 50 mL with acetone.
- Controls:
Blank: Dechlorinated tap water (carbon filter)
Vehicle: Dechlorinated tap water with the vehicle
- Chemical name of vehicle: Acetone and alkylphenol-polykol-ether
- Concentration of vehicle in test medium: 791 mg acetone and 0.4 mg alkylphenol-polyglykol-ether per liter water in the concentration used for the highest test concentration.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Length: 21 mm (15-25 mm)
- Weight: 0.11 (0.02-0.20g)
- Source: West Aquarium, Bad Lauterberg, Germany
- Feeding during test: None

ACCLIMATION
- Acclimation period: 19 days

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Hardness:
164 mg CaCO3/L
Test temperature:
22-24 °C
pH:
8.0-8.3
Dissolved oxygen:
O2%: 90-102
Nominal and measured concentrations:
Nominal concentrations: 10, 18, 32, 58, 100 mg/L;
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 L glass aquaria
- Material, fill volume: Glass aquaria filled with 15 L
- No. of organisms: 10 fishes per concentration and control, 10 fishes per aquarium



Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- No abnormal responses of the fish were observed.
- No mortality of the fish were observed.

Any other information on results incl. tables

   measurement  start  24h  48h  72h  96h
blank  dead/alive  0/10 0/10   0/10  0/10  0/10
 oxygen %  102  95  93  90  91
 pH  8.0  8.1  8.3  8.3  8.3
 temperature °C  22  22  23  23  23
Vehicle            dead/alive  0/10  0/10  0/10  0/10  0/10
 oxygen %  101  91  91  90  90
 pH  8.2  8.2  8.2  8.2  8.2
 temperature °C  23  23  23 23  23 
          10 mg/L  dead/alive  0/10 0/10  0/10  0/10  0/10 
 oxygene %  102 92 91  90  90 
 pH  8.0 8.2  8.2  8.2  8.2 
 temperature °C  22 23  23  23  23 
          18 mg/L  dead/alive  0/10 0/10  0/10  0/10  0/10 
 oxygen %  101  99 98  96  92 
 pH  8.1 8.1  8.2  8.2  8.2 
 temperature °C  22 23  23  23  23 
          32 mg/L  dead/alive  0/10 0/10  0/10  0/10  0/10 
 oxygene %  102 94  96  94  92 
 pH  8.1 8.2  8.3  8.3  8.3 
 temperature °C  22 23  23  23  23 
          58 mg/L  dead/alive  0/10 0/10  0/10  0/10  0/10 
 oxygen %  102 99  96  94  92 
 pH  8.0  8.2  8.2  8.2  8.2
 temperature °C  22 23  23  23  23 
          100 mg/L  dead/alive  0/10 0/10  0/10  0/10  0/10 
 oxygen %  102 96  93  92  90 
 pH  8.1 8.1  8.2  8.2  8.2 
 temperature °C  22 22  23  23  23 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance is with high probability not acutely harmful to fish.