Rose, Rosa damascena, ext.

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 5 to 12 September 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP certificate (inspected on 13 and 14 October 2014 / signed on 8 April 2015)

Type of method:

dynamic method

Remarks:

Cottrell pump is used in the main test (pre-test with Siwoloboff method is performed)

Key result

Atm. press.:

ca. 101 325 Pa

Decomposition:

yes

Remarks:

Considering the complex nature of the substance, the temperature of initial decomposition of the test item cannot be clearly determined using these methods. Using SIWOLOBOFF and dynamic method signs of decomposition were observed from 210°C.

Decomp. temp.:

>= 210 °C

Remarks on result:

not determinable

Remarks:

Decomposition

Pre-test :

Using Siwoloboff method, small bubbles were observed from approximately 120°C, condensate on the reagent glass wall was observed from approximately 170°C and change of colour was observed from approximately 210°C (red-orange to greenish). More and more bubbles were observed from 210°C to 247.2°C (turned off). In cooling down phase, no bubbles at the surface of the sample were observed at 239°C.

After cooling down the test item was greenish.

Results main test

Observations and measured values are presented in the following table.

Table 4.3.2.-     Results

Decomposition	Temperature Cottrell pump	Temperature test item	Atmospheric Pressure
First determination	225.3 °C (498.5 K)	234.0 °C (507.2 K)	99875 Pa
Second determination	225.7 °C (498.9 K)	234.0 °C (507.2 K)	99880 Pa

 

Table 4.3.3  .-Observations

Observations	Temperature Cottrell pump	Temperature test item
Lots of small bubbles	since 30 °C	since 90 °C
The colour was slightly greenish	since 210 °C	since 225°C

After cooling down the test item was greenish.

Boiling, indicated by pumping of the Cottrell pump, was observed at 225.3 °C (first determination) and 225.7°C (second determination), while the temperature measured in the sample was 234°C (between 99875 and 99880 Pa).

For this complex substance, the thermometer in the Cottrell pump showed a lower temperature than the temperature in the sample, which may be the sign of decomposition. In addition, the colour change of the test item after cooling down indicated clearly decomposition of the test item during the experiment.

Considering the complex nature of the substance, the temperature of initial decomposition of the test item cannot be clearly determined using these methods. However signs of decomposition of the substance (such as change of colour and difference between temperatures of the test item and in the Cottrell pump) were observed from 210°C.

As the test item showed decomposition, the calculation following the equation of Sydney – Young was not necessary.

No observations were made which might cause doubts on the validity of the study outcome. Therefore, the result of the study is considered valid.

Conclusions:

No boiling point was recorded due to decomposition of the substance.

Executive summary:

A study was conducted to determine the boiling point of the test substance under GLP according to OECD 103 / EU A2 guideline.

No boiling point was determined because of decomposition of the test item observed (colour change and temperature difference between test item and the Cottrell pump)

The temperature of initial decomposition of the substance is not clear. Condering the observations reported using Siwoloboff and Cottrell methods, the initial decomposition is considered to be observed from approximately 210°C (colour change).

According to Regulation (EC) No 1907/2006 (REACH) annex VII, column 2 of section 7.3, the determination of the boiling point does not need to be conducted for substances which decompose before boiling (e.g. auto-oxydation, rearrangement, degradation, decomposition, etc...)Therefore no additional test was conducted.

Description of key information

No boiling point was recorded due to the decomposition of the test item before boiling.

The temperature of initial decomposition of the test item cannot be clearly determined using SIWOLOBOFF and dynamic method.

However, using these methods, signs of decomposition of the substance (such as change of colour) were observed from 210°C.

Additional information

A reliable experimental study, conducted according to a recognized OECD/EC method and under GLP, is available. It is considered as a key study.