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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22 Sep 2003 to 20 Oct 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
Version / remarks:
OECD Guidelines for the Testing of Chemicals "Modified OECD Screening Test" Part 301 E, adopted July 1ih 1992
Deviations:
not specified
Qualifier:
according to
Guideline:
EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)
Version / remarks:
EU-Guideline C.4-B, adopted September 1993 "Part Ill: Modified OECD Screening Test"
Deviations:
not specified
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
No further details specified in the study report.
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Specification
Effluent from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.

Source
The effluent was taken from the effluent channel of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, lm Altenschemel, NW-Lachen-Speyerdorf. Date of taking: 22 Sep 2003
Duration of test (contact time):
28 d
Initial conc.:
69 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
CONDUCT OF THE STUDY
Preparations
The medium was prepared from the stock solutions. The inoculum was taken from its source and aerated.

Experimental Parameters
The test was performed with a start concentration of 40 mg carbon/l.
Two blanks, two flasks containing aniline as reference item, two flasks containing test item, one flask containing test item and 10 ml of a solution with 1% HgCI2 (without inoculum, testing for abiotic degradation), one flask containing test item and 10 ml of a solution with 1% HgCI2 (with inoculum, testing for adsorption on flasks or bacteria) and one flask containing test item and aniline (testing for toxic effects) were tested. Each flask except for the abiotic flask was inoculated with 0.5 ml effluent/l.
Each flask contained 200 mi.
The test was performed at room temperature (21 ± 1 °C) without direct lighting.
Start of the test was 22 Sep 2003
Duration of the test was 28 days.

Apparature
The test vessels were closed with perforated aluminium foil to enable aeration. They were put on an orbital shaker.

Sampling and Measurement
From each flask, samples (5 ml) were taken at day 0.125; 2; 4, 8; 14; 21 and 28. The samples were membrane filtrated and the DOC was measured. Measurement of DOC was performed following SOP 118 009 02 in the current edition, using a TOC5050A (SHJMADZU).
Reference substance:
aniline
Remarks:
A stock solution containing 2.1 g/l was prepared and its DOC measured.
Preliminary study:
Not performed.
Test performance:
The difference between replicates was <1 % (criterion ≤ 20).
The reference item reached the pass level on day 4. (criterion ≤ 14)
Key result
Parameter:
% degradation (DOC removal)
Value:
4.6
Sampling time:
28 d
Details on results:
The test item Glycerin-1-allylether can be considered as "not readily biodegradable".
The degree of biodegradation was below 10 % after 28 days.
No 10d-window could be detected.
Results with reference substance:
The reference item reached the pass level on day 4. (criterion ≤ 14)

DOC-values

Day

Blank 1

Blank 2

Ref 1

Test 1

Test 2

Abiot.

Ads.

Toxic.

0

2.842

2.194

38.13

38.73

39.52

39.31

39.27

76.05

2

1.951

1.578

37.99

40.81

40.62

40.83

40.91

79.31

4

1.619

1.367

1.944

38.52

39.71

39.53

39.14

39.23

8

3.517

0.669

2.728

39.16

39.12

39.24

38.73

41.26

14

0.809

0

1.786

3777

38.04

38.79

39.00

38.86

21

1.865

1.032

2.226

38.65

38.66

39.47

40.74

39.62

28

0.505

0.279

1.716

38.11

39.20

40.50

40.93

39.31

 

Table

Day

Ref 1

Test 1

Test 2

Test Mean

Abiot.

Ads.

Toxic.

2

-1.7

-7.8

-5.0

-6.4

-3.9

-2.6

-5.5

4

98.7

-2.3

-3.3

-2.8

-0.6

-1.8

48.7

8

98.2

-2.4

-0.1

-1.3

0.2

0.1

46.7

14

96.1

-3.2

-1.7

-2.5

1.3

-6.3

47.7

21

97.8

-2.7

-0.6

-1.7

-0.4

-6.5

48.1

28

96.3

-4.2

-4.9

-4.6

-3.0

-7.3

48.4

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Glycerin-1-allylether is not readily biodegradable following OECD 301 E/EU C.4-B.
Executive summary:

The test item was tested using a concentration of 40 mg organic carbon/L in test medium following OECD 301 E/EU Guideline C.4-B.

Aniline was chosen as reference item. Effluent from a sewage treating plant was used as inoculum (conc. 0.5 ml/L). The test was left running for 28 days.

To measure the degradation of test and reference item, the content of dissolved organic carbon (DOC) in the test vessels was measured seven times during the test.

All validity criteria were met. Degradation of the reference item was 99% after four days.

The following data could be determined for the test item Glycerin-1-allylether:

10-day-window: not detectable

degradation at the end of the test below 10 %

pass level: 70% at the end of 10-d-window

Therefore Glycerin-1-allylether is not readily biodegradable following OECD 301 E/EU C.4-B.

Description of key information

The following data could be determined for the test item Glycerin-1-allylether:

10-day-window: not detectable

degradation at the end of the test below 10 %

pass level: 70% at the end of 10-d-window

Therefore Glycerin-1-allylether is not readily biodegradable following OECD 301 E/EU C.4-B.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The test item was tested using a concentration of 40 mg organic carbon/L in test medium following OECD 301 E/EU Guideline C.4-B.

Aniline was chosen as reference item. Effluent from a sewage treating plant was used as inoculum (conc. 0.5 ml/L). The test was left running for 28 days.

To measure the degradation of test and reference item, the content of dissolved organic carbon (DOC) in the test vessels was measured seven times during the test.

All validity criteria were met. Degradation of the reference item was 99% after four days.

The following data could be determined for the test item Glycerin-1-allylether:

10-day-window: not detectable

degradation at the end of the test below 10 %

pass level: 70% at the end of 10-d-window

Therefore Glycerin-1-allylether is not readily biodegradable following OECD 301 E/EU C.4-B.