Triethylsilane

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998/01/19-1998/01/23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetramethylsilane

EC Number:

200-899-1

EC Name:

Tetramethylsilane

Cas Number:

75-76-3

Molecular formula:

C4H12Si

IUPAC Name:

tetramethylsilane

Test material form:

liquid

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS

- Source: Harlan, Winkelmann GmbH, 33176 Borchen, Gartenstrasse 27

- Age at study initiation: adult animals

- Weight at study initiation: 2.6-3.1 kg

- Housing: conventional, single housing in stainless steel cages

- Diet: Ssniff K 4 complete feed for rabbits, ad libitum. Supplied by Fa. Gelsenwasser, Wasserwerk, 45721 Haltern

- Water: Tap water, ad libitum

- Acclimation period:minimum 14 days


ENVIRONMENTAL CONDITIONS

- Temperature (°C): 20 +/- 3

- Humidity (%): 30-70

- Air changes (per hr): 15

- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: Untreated eye

Amount / concentration applied:

TEST MATERIAL

- Amount(s) applied (volume or weight with unit): 0.1 cm3

- Concentration (if solution): undiluted

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

1, 24, 48, 72 hours

Number of animals or in vitro replicates:

3 males

Details on study design:

REMOVAL OF TEST SUBSTANCE

- Washing (if done): with warm physiological saline

- Time after start of exposure: 24 and 48 h


SCORING SYSTEM: According to OECD


TOOL USED TO ASSESS SCORE: hand-slit lamp/ fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Remarks on result:

no indication of irritation

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

2

Remarks on result:

no indication of irritation

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

3

Remarks on result:

no indication of irritation

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Remarks on result:

no indication of irritation

Results 5

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 24/48/72 h

Score:

1

Max. score:

0

Reversibility:

fully reversible within: 24 hours

Irritant / corrosive response data:

The administration of the test substance caused, within one hour, some definitely hyperaemic (injected) blood vessels. 24 to 72 hours after administration of the test substance, the animals did not show any more irritation on eye and mucous membranes.

Other effects:

None reported.

Any other information on results incl. tables

All scores were zero at all times other than at 1 hour, when the score for conjunctival redness was 1 for all three animals.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In a reliable study conducted according to the appropriate OECD test guideline and in compliance with GLP, tetramethylsilane was found to be non-irritant to rabbit eyes.