Reaction products of diazotised Sodium hydrogen 4-amino-5-hydroxynaphthalene-2,7-disulphonate coupled with Resorcinol (1:1), coupled with diazotised 5-amino-2-anilinobenzenesulphonic acid (1:2), coupled with diazotised 4-nitroaniline (1:1), sodium salts

Short-term toxicity to aquatic invertebrates

Description of key information

EC₅₀ (48 h, Daphnia magna) > 37 mg/l (meas. (geom. mean); equivalent to 100 mg/L nominal concentration)

NOEC (48 h, Daphnia magna) ≥ 37 mg/L (meas. (geom. mean); equivalent to 100 mg/L nominal concentration)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

37 mg/L

Additional information

The following data were obtained for Similar Substance 01. It is expected that the Target substance will present similar effect levels to invertebrates. Justification for the use of a read-across approach is given in Section 13 of IUCLID.

A limit test was conducted to determine the acute toxicity of Similar Substance 01 to Daphnia magna, according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. The study was performed using the concentration of 100 mg/L (nominal). For the test concentration, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. The concentration showed no significant toxicity. Only 1 animal (5 %) was immobilised in the treatment group which can be stated as not significant. None of the animals were immobilised in the blank control. Potassium dichromate was used as a positive control in a current reference study to assure that the test conditions were reliable. At the beginning and at the end of the test, the content of SImilar Substance 01 in the test solutions was determined using a photometer method. At the beginning of the test, the measured concentration was 38 % of the nominal concentration; after 48 hours, the measured test concentration was 37 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.

Based on immobilisation of daphnia, the 48-hour and 24-hour EC₅₀values, as well as the 48 -hour lowest observed effect concentration (LOEC) value, were determined to be greater than 37 mg/L. The corresponding 48-hour no observed effect concentration (NOEC) was determined to be at least 37 mg/L. Concentrations were based on the mean geometric measured concentration of which 37 mg/L corresponded to a nominal value of 100 mg/L.