4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5). EC50 intoxication value was estimated to be 215.30 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) was likely to be not toxic to aquatic invertebrate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5). EC50 growth rate was estimated to be 121.927 mg/l for Desmodesmus subspicatus for 72 hrs duration. It was concluded that 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) was likely to be not toxic to aquatic algae.

Additional information

Short-term toxicity to aquatic invertebrates

Following studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5). EC50 intoxication value was estimated to be 215.30 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) was likely to be not toxic to aquatic invertebrate.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across Sulfanilic acid (CAS: 121-57-3) from the publication Chemosphere 1994, suggests that theTo study the effects of 4-aminobenzenesulfonic acid on aquatic invertebrate test was carried out for 24 hr.

The effective concentration EC50 value for short term toxicity to aquatic invertebrates for Sulfanilic acid (CAS: 121-57-3) in 24 hrs is 109.13 mg/l. It can be concluded from the value that the Sulphanilic acid is not toxic to the aquatic invertebrate.Based on the result, Sulfanilic acid (CAS: 121-57-3) was considered as "not classified" as per the CLP regulations.

Another supporting experimental study for the structurally similar read across2-aminoanthraquinone (CAS: 117-79-3) from the secondary source, suggests that theTo study the effects of 2-aminoanthraquinone on aquatic invertebrate short term toxicity test was carried out for 48 hrs. mobility was measured during the test.

Effective concentration EC50 to 50% of Daphnia magna when exposed to 2-aminoanthraquinone for 48 hr is 378 mg/L. It can be concluded from the intoxication value that the 2-aminoanthraquinone (CAS: 117-79-3) is not toxic to the aquatic inverbrateand can be considered as “not classified” as per the classification criteria for aquatic environment.

Thus based on the effect concentrations which is in the range 109.13 mg/l to 378 mg/l give the conclusion that test substance 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5). EC50 growth rate was estimated to be 121.927 mg/l for Desmodesmus subspicatus for 72 hrs duration. It was concluded that 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) was likely to be not toxic to aquatic algae.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across Ammonium 3-amino-2,5-dichlorobenzoate (CAS: 1076-46-6) from the publication Hyacinth control journal 1972, suggests that theToxicity to aquatic algae and cyanobacteria test was carried out to study the effects of Ammonium 3-amino-2,5-dichlorobenzoate on aquatic algae. Decreasing trend ofPopulation growth rate was measured during the test.

Effective concentration to 50% of Dunaliella tertiolecta when exposed to Ammonium 3-amino-2,5-dichlorobenzoate for 10 days is 4000 mg/L. It can be concluded from the value that the Ammonium 3-amino-2,5-dichlorobenzoate (CAS: 1076-46-6) is not toxic to the aquatic algaecan be considered as “not classified” as per the classification criteria for aquatic environment.

Another supporting experimental study for the structurally similar read across 4 -aminobenzenesulfonic acid (CAS no. 121 -57 -3) from the High Production Volume (HPV) Challenge Program 2016, also suggests that theShort term toxicity toScenedesmus subspicatus(green algae) study was carried out for 72 hrs. The study was performed according to other guideline DIN 38412 Part 9, 1992. The study was based on the effects of the test compound 4 -aminobenzenesulfonic acid (CAS no. 121 -57 -3) onScenedesmus subspicatus(green algae) in a static fresh water system. Scenedesmus subspicatuswas used as a test organism. Based on effect on growth rate of the test organismScenedesmus subspicatus(green algae), the 72 hr EC10 and EC50 value was determined to be 2.7 and 375 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance 4 -aminobenzenesulfonic acid can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

Thus based on the effect concentrations which is in the range 121.927 mg/l to 4000 mg/l give the conclusion that test substance 4-[(5-amino-3-methyl-1-phenyl-1H-pyrazol-4-yl)azo]-2,5-dichlorobenzenesulphonic acid (CAS: 12239-15-5) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.