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EC number: 203-247-4 | CAS number: 104-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05.01. - 08.01.2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-chlorobenzaldehyde
- EC Number:
- 203-247-4
- EC Name:
- 4-chlorobenzaldehyde
- Cas Number:
- 104-88-1
- Molecular formula:
- C7H5ClO
- IUPAC Name:
- 4-chlorobenzaldehyde
- Details on test material:
- Batch No.: DEBR006719
Purity (certified): 99.4 % (a/a) +/- 0.03 %
appearance: soldified melt, white
Expiry date: 2010-07-14
recommended storage: RT
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The test item concentrations were analytically determined from all test concentrations and the control after 0 h (test start) and 72 h (test end). Samples at start and end of the test were taken directly from the test replicates.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Stock solution: 200 mg/L, freshly prepared with dilution water
Dispersion treatment: 45 min magnetic stirring at 45°C and 1000 rpm
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Origin: Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, D-37073 Göttingen
Culture medium: Nutrient medium Z according to Lüttge et al. (1994)
Preculture: A four day old preculture was used as inoculum. Incubation was performed in 500 mL Erlenmeyer flasks with dilution water. For the start of the test the preculture was directly pipetted into each test concentration and the control.
Initial cell density: Approximately 2 - 5 x 103 cells/mL
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- The medium had a nominal hardness of 0.24 mmol Ca+Mg/L
- Test temperature:
- Nominal range: 21 – 22 °C
- pH:
- Test start: 8.0 - 8.1
Test end: 8.0 - 8.4 - Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Test concentrations: (nom.) 1.00 - 3.20 - 10.0 - 32.0 - 100 mg/L (factor: √10)
Test concentrations: 0.208 – 1.68 – 7.26 – 26.7 – 84.5 mg/L (factor: √10) (geometric mean measured) - Details on test conditions:
- TEST METHOD
Procedure: static
Exposure duration: 72 h
Replicates: Three replicates for each concentration level, six for the control
Test container: Sterile Erlenmeyer flasks, volume: 250 mL, sealed with cotton wool plugs.
Test volume: 25 mL
Dilution water: According to the guideline (OECD 201 medium)
This medium had a nominal hardness of 0.24 mmol Ca+Mg/L.
Preculture: A four day old preculture was used as inoculum. Incubation was performed in 500 mL Erlenmeyer flasks with dilution water. For the start of the test the preculture was directly pipetted into each test concentration and the control.
Initial cell density: Approximately 2 - 5 x 103 cells/mL
Application: Application was carried out by adding appropriate volumes of the stock solution into each test concentration.
Temperature: Nominal range: 21 – 24 °C, controlled at 2 °C
Agitation: Test containers were placed on a rotary shaker and oscillated at approximately 70 rpm.
Light intensity: 60 - 120 µE • m-2 • s-1
Light regime: 24 h/d light
TYPE AND FREQUENCY OF MEASUREMENT
Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal. The pH-value at the beginning of the test was measured out of one additional replicate from each concentration level and the control. At the end it was measured from one pooled replicates of each concentration level and the control. The room temperature was measured continuously by a hygro-thermograph. Light intensity was measured prior to test start. Microscopic evaluation of the cells at the start and end of the incubation was carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of algae cells.
STATISTICS
The NOEC and LOEC were determined by calculation of statistical significance of growth rates and yield. One Way Analysis of Variance was carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance a Normality Test and an Equal Variance Test were done first. P-values for both Normality and Equal Variance Tests were 0.05. The a-value for ANOVA was a=0.05. Normality Test and Equal Variance Test failed when running a One Way Analysis of Variance for growth rates. Transformation (y = 10^y) was carried out with the yield values.
EC10-, EC20- and EC50-values of the growth rate and yield inhibition after 72 h were calculated by sigmoidal dose-response regression. Calculation of the confidence intervals EC10-, EC20- and EC50-values were carried out using standard procedures. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 15.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 14.6 - 16.9
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.47 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 7.20 - 20.9
- Details on results:
- EC10-, EC20- and EC50-values of the growth rate and yield inhibition after 72 h were calculated by sigmoidal dose-response regression. Calculation of the confidence intervals EC10-, EC20- and EC50-values were carried out using standard procedures.
(see also "any other information on results incl. tables")
The nominal concentrations of 1.00 - 3.20 - 10.0 - 32.0 - 100mg/L corresponded to geometric mean measured test item concentrations of 0.208 – 1.68 – 7.26 – 26.7 – 84.5 mg/L. The concentrations of p-Chlorbenzaldehydand its metabolite chlorobenzoic acid were analysed at all concentration levels after 0 and 72 h via HPLC analysis. The measured concentrations of p-Chlorbenzaldehydat test start were in the range of 93 – 106 % of the nominal values. At the end of the test the measured concentrations of p-Chlorbenzaldehyd decreased whereas those of its metabolite chlorobenzoic acid increased correspondingly. The sum of p-Chlorbenzaldehydand its metabolite was calculated to be in the range of 4 – 83 % of the nominal values. - Results with reference substance (positive control):
- Last test was performed on September 14 to 17, 2009, test concentrations were 0.25, 0.50 and 1.0 mg/L.
ErC50 = 0.600
EyC50 = 0.280
Any other information on results incl. tables
NOEC, LOEC and EC - values and 95 % Confidence Intervals of p-Chlorbenzaldehyd(0-72 h)
based on geometric mean measured test item concentrations [mg/L]
|
Rate-related Inhibition |
NOEC |
1.68 |
LOEC |
7.26 |
ErC10 |
8.63 (7.67 – 9.74) |
ErC20 |
10.8 (9.71 – 11.9) |
ErC50 |
15.8 (14.6 – 16.9) |
|
Inhibition of Yield |
NOEC |
1.68 |
LOEC |
7.26 |
EyC10 |
6.14 (2.75 – 18.9) |
EyC20 |
6.92 (3.09 – 19.2) |
EyC50 |
8.49 (7.20 – 20.8) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The study was conducted under static conditions with an initial cell density of approximately 2 – 5 x 103cells/mL. Five concentration levels were tested in a geometrical series with a dilution factor of√10, nominal:1.00 - 3.20 - 10.0 - 32.0 - 100mg/L corresponding to geometric mean measured test item concentrations of 0.208 – 1.68 – 7.26 – 26.7 – 84.5 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. Environmental conditions were determined to be within the acceptable limits.
p-Chlorbenzaldehydwas found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 h with the following effect values (geometric mean measuredtest item concentrations): The EC50 -values with 95 % confidence intervals for inhibition of specific growth rate (ErC50) and yield (EyC50) after 72 h were 15.8 (14.6 – 16.9) and 8.49 (7.20 – 20.8) mg/L, respectively. All effect levels are given based on geometric mean measured concentrations of p-Chlorobenzaldehyd.
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