Lithium trifluoromethanesulphonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 05 June 2012 to 22 nov 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

11 april 2011

Test material

Test material information

Constituent 1

Reference substance name:

Trifluoromethanesulphonic acid

EC Number:

216-087-5

EC Name:

Trifluoromethanesulphonic acid

Cas Number:

1493-13-6

Molecular formula:

CHF3O3S

IUPAC Name:

trifluoromethanesulfonic acid

Test material form:

liquid

Details on test material:

See Confidential details on test material

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, industrial, adapted

Details on inoculum:

- Specification: Effluent from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.
- Source: The effluent was taken from the effluent channel of the sewage treatment plant, Edenkoben.
Date of collection: 19. Jun. 2012, batch no: 20120619.
- Pre-Treatment: The effluent was kept aerobic during transport and storage. Before usage, the effluent was left to settle for one hour. The effluent was aerated for five days at room temperature in order to lower the oxygen consumption of the blanks. 0.5 mL/L test medium were used.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

21.97 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
Stock solutions:
Solution A:
Potassium dihydrogenephosphate (KH2PO4): 8.5 g
Di-potassium hydrogenephosphate (K2HPO4): 21.75 g
Sodium di-hydrogenephosphate hydrate (NaH2PO4*H2O): 33.4 g
Ammonia chloride (NH4Cl): 0.5 g
H2O demin. ad: 1000 mL
The pH was adjusted to 7,4 +/- 0.1

Solution B:
Calcium chloride dihydrate (CaCl2*2H2O): 36.4 g
H2O demin. ad: 1000 mL

Solution C
Magnesium sulfate heptahydrate (MgSO4*7H2O): 22.5 g
H2O demin. ad: 1000 mL

Solution D
Iron(III) chloride hexahydrate (FeCl3*6H2O): 0.25 g
Di-sodium-ethylendiamintetraacetate dihydrate (Na2EDTA*2H2O): 0.4 g
H2O demin. ad : 1000 mL

The medium was freshly prepared.
Composition:
Solution A 1 mL
Solution B 1 mL
Solution C 1 mL
Solution D 1 mL
H2O demin. ad: 1000 mL

- Test temperature: 19.8 – 21.5°C
- Suspended solids concentration: 0.5 mL/L
- Continuous darkness: yes
- Other: After preparation, the medium was aerated for 50 hours. Then the medium was stored at room temperature for 20 hours without aeration. The O2-concentration was then determined with 8.63 mg/L at 21°C.

TEST SYSTEM
- Culturing apparatus: 250 mL narrow neck bottles with glass stoppers.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The effluent was taken from the sewage treatment plant and was aerated for five days in order to lower the oxygen demand of the effluent.
- Measuring equipment: Oximeter 538 wtw (for oxygen)

SAMPLING
- Sampling frequency: For each test series, seven samplings were made (on days 0, 3, 7, 11, 18, 23 and 28). For each sampling, the oxygen content of two vessels of each test series was measured (one vessel on day 0).


CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Positive control: 2
- Toxicity control: 2

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

3.61 mg/L

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

For the determination of the biological results, the data were evaluated without consideration of nitrification. Because neither test item nor positive control contains nitrogen, the O2-uptake caused by nitrification was not considered for the determination of the degradation.
• The test item Trifluoromethanesulfonic acid must be considered as “not readily biodegradable“.
• The degree of biodegradation was 0 % after 28 days
• A 10-day-window could not be detected.

BOD5 / COD results

Results with reference substance:

The positive control reached the pass level already on day 3.

Any other information on results incl. tables

Degradation without Consideration of Nitrification in %:

Day	Positive Control			Test Item			Toxicity Control
	1	2	Mean	1	2	Mean	1	2	Mean
3	68.5	67.5	68.0	-6.7	-6.6	-6.6	-7.3	-7.0	-7.2
7	78.4	80.6	79.5	-1.4	-1.0	-1.2	38.0	37.7	37.9
11	82.4	82.2	82.3	-1.1	-2.0	-1.6	36.5	36.2	36.3
18	77.7	80.7	79.2	-1.5	-2.0	-1.7	38.8	37.0	37.9
23	79.4	74.1	76.8	-7.2	-7.3	-7.2	29.7	31.0	30.4
28	83.3	81.3	82.3	-1.7	-0.9	-1.3	35.8	36.4	36.1

Negative degradation values are caused by measurement uncertainties resp. small differences between oxygen decrease in the controls and in the test flasks.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

O2-consumption in the controls: =< 1.5 mg/L Lowest measured O2-concentration in the test vessels: >= 0.5 mg/L Difference within replicates: =< 20% Degradation of positive control > 60%: =< 14 days Degradation in the toxicity control on day 14: >= 25%

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Trifluoromethanesulfonic acid is not readily biodegradable in the closed bottle test.

Executive summary:

The study was performed in order to evaluate the aerobic ultimate biodegradation potential of Trifluoromethanesulfonic acid in a test for ready biodegradability, according to the OECD 301D guideline and under GLP conditions.

This study used a test item concentration of 21.97 mg/L (corresponding to a theoretical oxygen consumption of 5.86 mg/L).

The solution of the test item in mineral medium was inoculated with a relatively small number of micro-organisms from a mixed population and kept in completely full, closed bottles in the dark at constant temperature.

Degradation was followed by analysis of dissolved oxygen over a 28-day period. The amount of oxygen taken up by the microbial population during biodegradation of the test item, corrected for uptake by the blank inoculum run in parallel, is expressed as a percentage of COD.

There was 0% of degradation of test item after 28 days. The positive control reached the pass level already on day 3.As degradation in the toxicity flask was more than 25 % at the end of the test, the test item can be stated as not toxic towards the inoculum in a concentration of 11.72 mg/L

In these test conditions, Trifluoromethanesulfonic acid was not readily biodegradable.